Anti-SEC61B Antibody (CAB15788)[KO Validated]
![Anti-SEC61B Antibody (CAB15788)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/21878/21730/anti-sec61b-antibody-cab15788ko-validated__29282__40181.1645805020.jpg?c=1 "Anti-SEC61B Antibody (CAB15788)[KO Validated]")
- SKU:
- CAB15788
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
![Anti-SEC61B Antibody (CAB15788)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/21878/21730/anti-sec61b-antibody-cab15788ko-validated__29282__40181.1645805020.jpg?c=1&imbypass=on "Anti-SEC61B Antibody (CAB15788)[KO Validated]")
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Description
| 抗体名: | Anti-SEC61B Antibody [KO Validated] |
| 抗体コード: | CAB15788 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T, HeLa, HepG2, SH-SY5Y, mouse brain, mouse stomach |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MPGP TPSG TNVG SSGR SPSK AVAA RAAG STVR QRKN ASCG TRSA GRTT SAGT GGMW RFYT EDSP GLKV GPVP VLVM SLLF IASV FMLH IWGK YTRS |
| 遺伝子ID: | 10952 |
| Uniprot: | P60468 |
| セルラーロケーション: | Endoplasmic reticulum membrane, Single-pass membrane protein |
| 計算された分子量: | 9kDa |
| 観察された分子量: | 12kDa |
| 同義語: | SEC61B |
| バックグラウンド: | The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. |
| UniProt Protein Function: | SEC61B: Necessary for protein translocation in the endoplasmic reticulum. Belongs to the SEC61-beta family. |
| UniProt Protein Details: | Protein type:Endoplasmic reticulum; Membrane protein, integral Chromosomal Location of Human Ortholog: 9q22.33 Cellular Component: membrane; endoplasmic reticulum; integral to membrane Molecular Function:protein binding; epidermal growth factor binding Biological Process: retrograde protein transport, ER to cytosol; SRP-dependent cotranslational protein targeting to membrane; antigen processing and presentation of peptide antigen via MHC class I; ER-associated protein catabolic process; cellular protein metabolic process; translation; protein import into nucleus, translocation; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression |
| NCBI Summary: | The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P60468 |
| NCBI GenInfo Identifier: | 42560366 |
| NCBI Gene ID: | 10952 |
| NCBI Accession: | P60468.2 |
| UniProt Related Accession: | P60468 |
| Molecular Weight: | |
| NCBI Full Name: | Protein transport protein Sec61 subunit beta |
| NCBI Synonym Full Names: | SEC61 translocon beta subunit |
| NCBI Official Symbol: | SEC61B |
| NCBI Protein Information: | protein transport protein Sec61 subunit beta |
| UniProt Protein Name: | Protein transport protein Sec61 subunit beta |
| UniProt Gene Name: | SEC61B |
| UniProt Entry Name: | SC61B_HUMAN |
![Anti-SEC61B Antibody [KO Validated] (CAB15788)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/f/311/A15788_1__85071.jpg) | Western blot analysis of extracts of various cell lines, using SEC61B antibody (CAB15788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2s. |
![Anti-SEC61B Antibody [KO Validated] (CAB15788)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/m/436/A15788_2__61091.jpg) | Western blot analysis of extracts from normal (control) and SEC61B knockout (KO) 293T cells, using SEC61B antibody (CAB15788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
![Anti-SEC61B Antibody [KO Validated] (CAB15788)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/n/339/A15788_3__74968.jpg) | Immunofluorescence analysis of L929 cells using SEC61B antibody (CAB15788) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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