Anti-SCYL2 Antibody (CAB13178)
- SKU:
- CAB13178
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SCYL2 Antibody |
| 抗体コード: | CAB13178 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SCYL2 (NP_060458.3). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse brain, Mouse heart, Mouse kidney, Rat brain, Rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SCYL2 (NP_060458.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MESM LNKL KSTV TKVT ADVT SAVM GNPV TREF DVGR HIAS GGNG LAWK IFNG TKKS TKQE VAVF VFDK KLID KYQK FEKD QIID SLKR GVQQ LTRL RHPR LLTV QHPL EESR DCLA FCTE PVFA SLAN VLGN WENL PSPI SPDI KDYK LYDV ETKY GLLQ VSEG LSFL HSSV KMVH GNIT PENI ILNK SGAW KIMG FDFC VSST NPSE QEPK FPCK EWDP NLPS LCLP NPEY LAPE YILS VSCE TASD MYSL GTVM YAVF NKGK PIFE VNKQ DIYK SFSR QLDQ LSRL GSSS LTNI PEEV |
| 遺伝子ID: | 55681 |
| Uniprot: | Q6P3W7 |
| セルラーロケーション: | Cytoplasm, Cytoplasmic vesicle, Endosome membrane, Golgi apparatus, clathrin-coated vesicle, perinuclear region, trans-Golgi network membrane |
| 計算された分子量: | 103kDa |
| 観察された分子量: | 104kDa |
| 同義語: | SCYL2, CVAK104 |
| バックグラウンド: | The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | SCYL2: Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L- lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system. Belongs to the protein kinase superfamily. |
| UniProt Protein Details: | Protein type:Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; SCY1 family Chromosomal Location of Human Ortholog: 12q23.1 Molecular Function:protein binding; receptor binding Biological Process: endosome to lysosome transport; positive regulation of receptor internalization |
| NCBI Summary: | The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015] |
| UniProt Code: | Q6P3W7 |
| NCBI GenInfo Identifier: | 74762350 |
| NCBI Gene ID: | 55681 |
| NCBI Accession: | Q6P3W7.1 |
| UniProt Secondary Accession: | Q6P3W7,Q96EF4, Q96ST4, Q9H7V5, Q9NVH3, Q9P2I7, A8KAB5 |
| UniProt Related Accession: | Q6P3W7 |
| Molecular Weight: | 103,709 Da |
| NCBI Full Name: | SCY1-like protein 2 |
| NCBI Synonym Full Names: | SCY1 like pseudokinase 2 |
| NCBI Official Symbol: | SCYL2 |
| NCBI Official Synonym Symbols: | CVAK104 |
| NCBI Protein Information: | SCY1-like protein 2 |
| UniProt Protein Name: | SCY1-like protein 2 |
| UniProt Synonym Protein Names: | Coated vesicle-associated kinase of 104 kDa |
| Protein Family: | SCY1-like protein |
| UniProt Gene Name: | SCYL2 |
| UniProt Entry Name: | SCYL2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SCYL2 antibody (CAB13178) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of L929 cells using SCYL2 Rabbit pAb (CAB13178) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using SCYL2 Rabbit pAb (CAB13178) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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