Anti-SBF1 Antibody (CAB12354)
- SKU:
- CAB12354
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-SBF1 Antibody |
| 抗体コード: | CAB12354 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1770 of human SBF1 (NP_002963.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | Raji, Jurkat, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1770 of human SBF1 (NP_002963.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | PPYD WELA QGPP EPPE EERS DGGA PQSR RRVV WPCY DSCP RAQP DAIS RLLE ELQR LETE LGQP AERW KDTW DRVK AAQR LEGR PDGR GTPS SLLV STAP HHRR SLGV YLQE GPVG STLS LSLD SDQS SGST TSGS RQAA R |
| 遺伝子ID: | 6305 |
| Uniprot: | O95248 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 208kDa/211kDa |
| 観察された分子量: | 208kDa |
| 同義語: | SBF1, CMT4B3, DENND7A, MTMR5 |
| バックグラウンド: | This gene encodes a member of the protein-tyrosine phosphatase family. However, the encoded protein does not appear to be a catalytically active phosphatase because it lacks several amino acids in the catalytic pocket. This protein contains a Guanine nucleotide exchange factor (GEF) domain which is necessary for its role in growth and differentiation. Mutations in this gene have been associated with Charcot-Marie-Tooth disease 4B3. Pseudogenes of this gene have been defined on chromosomes 1 and 8. |
| UniProt Protein Function: | MTMR5: a pseudophosphatase and nuclear guanine nucleotide exchange factor (GEF) that activates RAB28, a small G protein of the Rab subfamily. Lacks several amino acids in the catalytic pocket, which renders it catalytically inactive as a phosphatase. The pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts. Defects in MTMR5 are the cause of Charcot-Marie-Tooth disease type 4B3 (CMT4B3). Charcot-Marie-Tooth disease is the most common inherited disorder of the peripheral nervous system. Interacts with the SET domain of MLL1. Interacts with SUV39H1. Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. Two isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GEF; GEF, Rab; Inhibitor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 22q13.33 Cellular Component: cytosol; integral to membrane Molecular Function:protein tyrosine/serine/threonine phosphatase activity; Rab guanyl-nucleotide exchange factor activity Biological Process: protein amino acid dephosphorylation Disease: Charcot-marie-tooth Disease, Type 4b3 |
| NCBI Summary: | This gene encodes a member of the protein-tyrosine phosphatase family. However, the encoded protein does not appear to be a catalytically active phosphatase because it lacks several amino acids in the catalytic pocket. This protein contains a Guanine nucleotide exchange factor (GEF) domain which is necessary for its role in growth and differentiation. Mutations in this gene have been associated with Charcot-Marie-Tooth disease 4B3. Pseudogenes of this gene have been defined on chromosomes 1 and 8. [provided by RefSeq, Dec 2014] |
| UniProt Code: | O95248 |
| NCBI GenInfo Identifier: | 158515405 |
| NCBI Gene ID: | 6305 |
| NCBI Accession: | O95248.3 |
| UniProt Secondary Accession: | O95248,O60228, Q5JXD8, Q5PPM2, Q96GR9, Q9UGB8, A6PVG9 |
| UniProt Related Accession: | O95248 |
| Molecular Weight: | 208kDa |
| NCBI Full Name: | Myotubularin-related protein 5 |
| NCBI Synonym Full Names: | SET binding factor 1 |
| NCBI Official Symbol: | SBF1 |
| NCBI Official Synonym Symbols: | MTMR5; CMT4B3; DENND7A |
| NCBI Protein Information: | myotubularin-related protein 5 |
| UniProt Protein Name: | Myotubularin-related protein 5 |
| UniProt Synonym Protein Names: | SET-binding factor 1; Sbf1 |
| UniProt Gene Name: | SBF1 |
 | Western blot analysis of extracts of various cell lines, using SBF1 antibody (CAB12354) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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