Anti-S1PR1 Antibody (CAB12935)
- SKU:
- CAB12935
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Frequently bought together:
Description
| 抗体名: | Anti-S1PR1 Antibody |
| 抗体コード: | CAB12935 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 303-382 of human S1PR1 (NP_001391.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | NIH/3T3, SH-SY5Y, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 303-382 of human S1PR1 (NP_001391.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | NSGT NPII YTLT NKEM RRAF IRIM SCCK CPSG DSAG KFKR PIIA GMEF SRSK SDNS SHPQ KDEG DNPE TIMS SGNV NSSS |
| 遺伝子ID: | 1901 |
| Uniprot: | P21453 |
| セルラーロケーション: | Cell membrane, Endosome, Membrane raft, Multi-pass membrane protein |
| 計算された分子量: | 42kDa |
| 観察された分子量: | 43kDa |
| 同義語: | S1PR1, CD363, CHEDG1, D1S3362, ECGF1, EDG-1, EDG1, S1P1 |
| バックグラウンド: | The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | EDG-1: a G protein-coupled receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Highly expressed in endothelial cells and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. Suggested to be involved in the processes that regulate the migration/differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. S1P-induced endothelial cell migration requires AKT1-mediated phosphorylation of the third intracellular loop. Seems to be coupled to the G(i) subclass of heteromeric G proteins. |
| UniProt Protein Details: | Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 1p21.2 Cellular Component: intracellular membrane-bound organelle; intrinsic to plasma membrane; nucleoplasm; plasma membrane Molecular Function:G-protein coupled receptor activity; G-protein-coupled receptor binding Biological Process: actin cytoskeleton reorganization; blood vessel maturation; cell adhesion; cell migration; chemotaxis; G-protein coupled receptor protein signaling pathway; lamellipodium biogenesis; regulation of bone mineralization; regulation of bone resorption |
| NCBI Summary: | The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
| UniProt Code: | P21453 |
| NCBI GenInfo Identifier: | 205371820 |
| NCBI Gene ID: | 1901 |
| NCBI Accession: | P21453.2 |
| UniProt Secondary Accession: | P21453,Q9BYY4, Q9NYN8, D3DT66, |
| UniProt Related Accession: | P21453 |
| Molecular Weight: | |
| NCBI Full Name: | Sphingosine 1-phosphate receptor 1 |
| NCBI Synonym Full Names: | sphingosine-1-phosphate receptor 1 |
| NCBI Official Symbol: | S1PR1 |
| NCBI Official Synonym Symbols: | EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362 |
| NCBI Protein Information: | sphingosine 1-phosphate receptor 1 |
| UniProt Protein Name: | Sphingosine 1-phosphate receptor 1 |
| UniProt Synonym Protein Names: | Endothelial differentiation G-protein coupled receptor 1; Sphingosine 1-phosphate receptor Edg-1; S1P receptor Edg-1; CD_antigen: CD363 |
| Protein Family: | Sphingosine 1-phosphate receptor |
| UniProt Gene Name: | S1PR1 |
 | Western blot analysis of extracts of various cell lines, using S1PR1 antibody (CAB12935) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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