Anti-RPN1 Antibody (CAB12497)
- SKU:
- CAB12497
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-RPN1 Antibody |
| 抗体コード: | CAB12497 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HL-60, HepG2, SKOV3, MCF7, BxPC-3, Mouse liver, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | APGS ASSE APPL INED VKRT VDLS SHLA KVTA EVVL AHLG GGST SRAT SFLL ALEP ELEA RLAH LGVQ VKGE DEEE NNLE VRET KIKG KSGR FFTV KLPV ALDP GAKI SVIV ETVY THVL HPYP TQIT QSEK QFVV FEGN HYFY SPYP TKTQ TMRV KLAS RNVE SYTK LGNP TRSE DLLD YGPF RDVP AYSQ DTFK VHYE NNSP FLTI TSMT RVIE VSHW GNIA VEEN VDLK HTGA VLKG PFSR YDYQ RQPD SGIS SIRS F |
| 遺伝子ID: | 6184 |
| Uniprot: | P04843 |
| セルラーロケーション: | Endoplasmic reticulum membrane, Melanosome, Single-pass type I membrane protein |
| 計算された分子量: | 68kDa |
| 観察された分子量: | 68kDa |
| 同義語: | RPN1, OST1, RBPH1 |
| バックグラウンド: | This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. |
| UniProt Protein Function: | RPN1: Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Belongs to the OST1 family. |
| UniProt Protein Details: | Protein type:EC 2.4.99.18; Glycan Metabolism - N-glycan biosynthesis; Transferase; Membrane protein, integral Chromosomal Location of Human Ortholog: 3q21.3 Cellular Component: endoplasmic reticulum membrane; membrane; rough endoplasmic reticulum; oligosaccharyl transferase complex; integral to membrane; melanosome Molecular Function:protein binding; dolichyl-diphosphooligosaccharide-protein glycotransferase activity Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; protein modification process; protein amino acid N-linked glycosylation via asparagine; gene expression; post-translational protein modification |
| NCBI Summary: | This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P04843 |
| NCBI GenInfo Identifier: | 132559 |
| NCBI Gene ID: | 6184 |
| NCBI Accession: | P04843.1 |
| UniProt Secondary Accession: | P04843,Q68DT1, B2R5Z0, D3DNB6, |
| UniProt Related Accession: | P04843 |
| Molecular Weight: | 607 |
| NCBI Full Name: | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 |
| NCBI Synonym Full Names: | ribophorin I |
| NCBI Official Symbol: | RPN1 |
| NCBI Official Synonym Symbols: | OST1; RBPH1 |
| NCBI Protein Information: | dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; RPN-I; ribophorin-1; oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit |
| UniProt Protein Name: | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 |
| UniProt Synonym Protein Names: | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Ribophorin I; RPN-I; Ribophorin-1 |
| Protein Family: | 26S proteasome regulatory |
| UniProt Gene Name: | RPN1 |
| UniProt Entry Name: | RPN1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPN1 antibody (CAB12497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody (CAB12497) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human tonsil using RPN1 antibody (CAB12497) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using RPN1 antibody (CAB12497) at dilution of 1:100 (40x lens). |
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