Anti-RPL34 Antibody (CAB15716)
- SKU:
- CAB15716
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-RPL34 Antibody |
| 抗体コード: | CAB15716 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human RPL34 (NP_000986.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Mouse brain, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human RPL34 (NP_000986.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MVQR LTYR RRLS YNTA SNKT RLSR TPGN RIVY LYTK KVGK APKS ACGV CPGR LRGV RAVR PKVL MRLS KTKK HVSR AYGG SMCA KCVR DRIK RAFL IEEQ KIVV KVLK AQAQ SQKA K |
| 遺伝子ID: | 6164 |
| Uniprot: | P49207 |
| セルラーロケーション: | |
| 計算された分子量: | 13kDa |
| 観察された分子量: | 13kDa |
| 同義語: | RPL34, L34 |
| バックグラウンド: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Overexpression of this gene has been observed in some cancer cells. Alternative splicing results in multiple transcript variants, all encoding the same isoform. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| UniProt Protein Function: | RPL34: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Transcript variants derived from alternative splicing, alternative transcription initiation sites, and/or alternative polyadenylation exist; these variants encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Translation; Ribosomal Chromosomal Location of Human Ortholog: 4q25 Cellular Component: cytoplasm; cytosol; nucleolus Molecular Function:RNA binding Biological Process: mRNA catabolic process, nonsense-mediated decay; ribosome biogenesis and assembly; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Overexpression of this gene has been observed in some cancer cells. Alternative splicing results in multiple transcript variants, all encoding the same isoform. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Feb 2016] |
| UniProt Code: | P49207 |
| NCBI GenInfo Identifier: | 22002070 |
| NCBI Gene ID: | 6164 |
| NCBI Accession: | P49207.3 |
| UniProt Secondary Accession: | P49207,Q6FG66, Q9BUZ2, |
| UniProt Related Accession: | P49207 |
| Molecular Weight: | 13,293 Da |
| NCBI Full Name: | 60S ribosomal protein L34 |
| NCBI Synonym Full Names: | ribosomal protein L34 |
| NCBI Official Symbol: | RPL34 |
| NCBI Official Synonym Symbols: | L34 |
| NCBI Protein Information: | 60S ribosomal protein L34 |
| UniProt Protein Name: | 60S ribosomal protein L34 |
| Protein Family: | 50S ribosomal protein |
| UniProt Gene Name: | RPL34 |
| UniProt Entry Name: | RL34_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPL34 antibody (CAB15716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
 | Immunofluorescence analysis of U-2 OS cells using RPL34 antibody (CAB15716) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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