Anti-RPL30 Antibody (CAB13690)
- SKU:
- CAB13690
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-RPL30 Antibody |
| 抗体コード: | CAB13690 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human RPL30 (NP_000980.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, MCF7, HepG2, A-549, Mouse brain, Mouse heart, Mouse kidney, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human RPL30 (NP_000980.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MVAA KKTK KSLE SINS RLQL VMKS GKYV LGYK QTLK MIRQ GKAK LVIL ANNC PALR KSEI EYYA MLAK TGVH HYSG NNIE LGTA CGKY YRVC TLAI IDPG DSDI IRSM PEQT GEK |
| 遺伝子ID: | 6156 |
| Uniprot: | P62888 |
| セルラーロケーション: | |
| 計算された分子量: | 12kDa |
| 観察された分子量: | 13kDa |
| 同義語: | RPL30, L30 |
| バックグラウンド: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| UniProt Protein Function: | RPL30: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 8q22.2 Cellular Component: cytosol; extracellular matrix; focal adhesion; membrane; nucleus Molecular Function:protein binding; RNA binding; structural constituent of ribosome Biological Process: defense response to Gram-negative bacterium; killing of cells of another organism; mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62888 |
| NCBI GenInfo Identifier: | 4506631 |
| NCBI Gene ID: | 6156 |
| NCBI Accession: | NP_000980.1 |
| UniProt Secondary Accession: | P62888,P04645, Q502Z6, B2R591, |
| UniProt Related Accession: | P62888 |
| Molecular Weight: | 40.2kD |
| NCBI Full Name: | 60S ribosomal protein L30 |
| NCBI Synonym Full Names: | ribosomal protein L30 |
| NCBI Official Symbol: | RPL30 |
| NCBI Official Synonym Symbols: | L30 |
| NCBI Protein Information: | 60S ribosomal protein L30 |
| UniProt Protein Name: | 60S ribosomal protein L30 |
| UniProt Synonym Protein Names: | Large ribosomal subunit protein eL30 |
| Protein Family: | 60S ribosomal protein |
| UniProt Gene Name: | RPL30 |
 | Western blot analysis of extracts of various cell lines, using RPL30 antibody (CAB13690) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat heart using RPL30 antibody (CAB13690) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using RPL30 antibody (CAB13690) at dilution of 1:100 (40x lens). |
 | Confocal immunofluorescence analysis of HeLa cells using RPL30 antibody (CAB13690) at dilution of 1:200. Blue: DAPI for nuclear staining. |
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