Anti-RPL22 Antibody (CAB9202)
- SKU:
- CAB9202
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-RPL22 Antibody |
| 抗体コード: | CAB9202 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human RPL22 (NP_000974.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, U-87MG, HepG2, Jurkat |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human RPL22 (NP_000974.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAPV KKLV VKGG KKKK QVLK FTLD CTHP VEDG IMDA ANFE QFLQ ERIK VNGK AGNL GGGV VTIE RSKS KITV TSEV PFSK RYLK YLTK KYLK KNNL RDWL RVVA NSKE SYEL RYFQ INQD EE |
| 遺伝子ID: | 6146 |
| Uniprot: | P35268 |
| セルラーロケーション: | |
| 計算された分子量: | 14kDa |
| 観察された分子量: | 15kDa |
| 同義語: | RPL22, EAP, HBP15, HBP15/L22, L22 |
| バックグラウンド: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. |
| UniProt Protein Function: | RPL22: a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:RNA-binding Chromosomal Location of Human Ortholog: 1p36.31 Cellular Component: focal adhesion; cytoplasm; ribonucleoprotein complex; nucleus; cytosol Molecular Function:heparin binding; protein binding; structural constituent of ribosome; RNA binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; alpha-beta T cell differentiation; gene expression; viral transcription; translational termination; viral infectious cycle |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35268 |
| NCBI GenInfo Identifier: | 4506613 |
| NCBI Gene ID: | 6146 |
| NCBI Accession: | NP_000974.1 |
| UniProt Secondary Accession: | P35268,Q6IBD1, B2R495, |
| UniProt Related Accession: | P35268 |
| Molecular Weight: | |
| NCBI Full Name: | 60S ribosomal protein L22 proprotein |
| NCBI Synonym Full Names: | ribosomal protein L22 |
| NCBI Official Symbol: | RPL22 |
| NCBI Official Synonym Symbols: | EAP; L22; HBP15; HBP15/L22 |
| NCBI Protein Information: | 60S ribosomal protein L22 |
| UniProt Protein Name: | 60S ribosomal protein L22 |
| UniProt Synonym Protein Names: | EBER-associated protein; EAP; Epstein-Barr virus small RNA-associated protein; Heparin-binding protein HBp15 |
| Protein Family: | 50S ribosomal protein |
| UniProt Gene Name: | RPL22 |
| UniProt Entry Name: | RL22_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPL22 antibody (CAB9202) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat lung using RPL22 antibody (CAB9202) at dilution of 1:100 (40x lens). |
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