Anti-RPL22 抗体 (CAB19866)

抗体名:	Anti-RPL22 Antibody [KO Validated]
抗体コード:	CAB19866
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant protein of human RPL22.

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
反応性:	Human, Mouse, Rat
ポジティブサンプル:	293T

免疫原:	Recombinant protein of human RPL22.
精製方法:	Affinity purification
ストレージバッファ:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	Email for sequence
遺伝子ID:	6146
Uniprot:	P35268
セルラーロケーション:
計算された分子量:	14kDa
観察された分子量:	16KDa

Anti-RPL22 Antibody [KO Validated] (CAB19866)

Western blot analysis of extracts from normal (control) and RPL22 knockout (KO) 293T cells, using RPL22 antibody (CAB19866) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90S.

同義語:

RPL22, EAP, HBP15, HBP15/L22, L22

バックグラウンド:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2.

UniProt Protein Function:	RPL22: a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:RNA-binding Chromosomal Location of Human Ortholog: 1p36.31 Cellular Component: focal adhesion; cytoplasm; ribonucleoprotein complex; nucleus; cytosol Molecular Function:heparin binding; protein binding; structural constituent of ribosome; RNA binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; alpha-beta T cell differentiation; gene expression; viral transcription; translational termination; viral infectious cycle
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008]
UniProt Code:	P35268
NCBI GenInfo Identifier:	4506613
NCBI Gene ID:	6146
NCBI Accession:	NP_000974.1
UniProt Secondary Accession:	P35268,Q6IBD1, B2R495,
UniProt Related Accession:	P35268
Molecular Weight:
NCBI Full Name:	60S ribosomal protein L22 proprotein
NCBI Synonym Full Names:	ribosomal protein L22
NCBI Official Symbol:	RPL22
NCBI Official Synonym Symbols:	EAP; L22; HBP15; HBP15/L22
NCBI Protein Information:	60S ribosomal protein L22
UniProt Protein Name:	60S ribosomal protein L22
UniProt Synonym Protein Names:	EBER-associated protein; EAP; Epstein-Barr virus small RNA-associated protein; Heparin-binding protein HBp15
Protein Family:	50S ribosomal protein
UniProt Gene Name:	RPL22
UniProt Entry Name:	RL22_HUMAN

Antibodies

Anti-RPL22 Antibody (CAB9202)

RPL22 Antibody (PACO04943)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)