Anti-RPL21 抗体 (CAB14254)

抗体名:	Anti-RPL21 Antibody
抗体コード:	CAB14254
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human RPL21 (NP_000973.2).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
反応性:	Human, Mouse, Rat
ポジティブサンプル:	HT-29, Raji, BxPC-3, DU145, Mouse liver

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human RPL21 (NP_000973.2).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	MTNT KGKR RGTR YMFS RPFR KHGV VPLA TYMR IYKK GDIV DIKG MGTV QKGM PHKC YHGK TGRV YNVT QHAV GIVV NKQV KGKI LAKR INVR IEHI KHSK SRDS FLKR VKEN DQKK KEAK EKGT WVQL KRQP APPR EAHF VRTN GKEP ELLE PIPY EFMA
遺伝子ID:	6144
Uniprot:	P46778
セルラーロケーション:
計算された分子量:	18kDa
観察された分子量:	18kDa

同義語:

RPL21, HYPT12, L21

バックグラウンド:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL21: Defects in RPL21 are a cause of generalized hypotrichosis simplex (HTS). A rare form of non-syndromic hereditary hypotrichosis without characteristic hair shaft anomalies. Affected individuals typically show normal hair at birth, but hair loss and thinning of the hair shaft start during early childhood and progress with age. Belongs to the ribosomal protein L21e family.
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 13q12.2 Cellular Component: membrane; cytoplasm; nucleolus; cytosol Molecular Function:structural constituent of ribosome; RNA binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; translational termination; viral infectious cycle Disease: Hypotrichosis 12
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P46778
NCBI GenInfo Identifier:	18104948
NCBI Gene ID:	6144
NCBI Accession:	NP_000973.2
UniProt Secondary Accession:	P46778,Q16699,
UniProt Related Accession:	P46778
Molecular Weight:	18,565 Da
NCBI Full Name:	60S ribosomal protein L21
NCBI Synonym Full Names:	ribosomal protein L21
NCBI Official Symbol:	RPL21
NCBI Official Synonym Symbols:	L21; HYPT12
NCBI Protein Information:	60S ribosomal protein L21
UniProt Protein Name:	60S ribosomal protein L21
Protein Family:	50S ribosomal protein
UniProt Gene Name:	RPL21
UniProt Entry Name:	RL21_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL21 antibody (CAB14254) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat brain using RPL21 antibody (CAB14254) at dilution of 1:100 (40x lens).
	Confocal immunofluorescence analysis of HeLa cells using RPL21 antibody (CAB14254) at dilution of 1:200. Blue: DAPI for nuclear staining.
	Confocal immunofluorescence analysis of A-431 cells using RPL21 antibody (CAB14254) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

RPL21 Antibody (PACO11915)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)