Anti-RPL14 Antibody (CAB6724)
- SKU:
- CAB6724
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-RPL14 Antibody |
| 抗体コード: | CAB6724 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RPL14 (NP_003964.3). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HL-60, HeLa, 293T, Jurkat, HepG2, Mouse ovary |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RPL14 (NP_003964.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MVFR RFVE VGRV AYVS FGPH AGKL VAIV DVID QNRA LVDG PCTQ VRRQ AMPF KCMQ LTDF ILKF PHSA HQKY VRQA WQKA DINT KWAA TRWA KKIE ARER KAKM TDFD RFKV MKAK KMRN RIIK NEVK KLQK AALL KASP KKAP GTKG TAAA AAAA AAAK VPAK KITA ASKK APAQ KVPA QKAT GQKA APAP KAQK GQKA PAQK APAP KASG KKA |
| 遺伝子ID: | 9045 |
| Uniprot: | P50914 |
| セルラーロケーション: | |
| 計算された分子量: | 23kDa |
| 観察された分子量: | 26kDa |
| 同義語: | RPL14, CAG-ISL-7, CTG-B33, L14, RL14, hRL14 |
| バックグラウンド: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| UniProt Protein Function: | RPL14: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 3p22-p21.2 Cellular Component: membrane; cytoplasm; cytosol Molecular Function:protein binding; structural constituent of ribosome; RNA binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; translational termination; ribosomal large subunit biogenesis and assembly; viral infectious cycle; rRNA processing |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50914 |
| NCBI GenInfo Identifier: | 212276521 |
| NCBI Gene ID: | 9045 |
| NCBI Accession: | P50914.4 |
| UniProt Related Accession: | P50914 |
| Molecular Weight: | 23kDa |
| NCBI Full Name: | 60S ribosomal protein L14 |
| NCBI Synonym Full Names: | ribosomal protein L14 |
| NCBI Official Symbol: | RPL14 |
| NCBI Official Synonym Symbols: | L14; RL14; hRL14; CTG-B33; CAG-ISL-7 |
| NCBI Protein Information: | 60S ribosomal protein L14 |
| UniProt Protein Name: | 60S ribosomal protein L14 |
| UniProt Synonym Protein Names: | CAG-ISL 7 |
| Protein Family: | 50S ribosomal protein |
| UniProt Gene Name: | RPL14 |
| UniProt Entry Name: | RL14_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPL14 antibody (CAB6724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat liver using RPL14 antibody (CAB6724) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF7 cells using RPL14 antibody (CAB6724). Blue: DAPI for nuclear staining. |
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