Anti-RLIM Antibody (CAB15837)
- SKU:
- CAB15837
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-RLIM Antibody |
| 抗体コード: | CAB15837 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 535-624 of human RLIM (NP_057204.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Raji, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 535-624 of human RLIM (NP_057204.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | FLLN EDDD DQPR GLTK EQID NLAM RSFG ENDA LKTC SVCI TEYT EGNK LRKL PCSH EYHV HCID RWLS ENST CPIC RRAV LASG NRES VV |
| 遺伝子ID: | 51132 |
| Uniprot: | Q9NVW2 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 52kDa/68kDa |
| 観察された分子量: | 75kDa |
| 同義語: | RLIM, MRX61, NY-REN-43, RNF12 |
| バックグラウンド: | The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| UniProt Protein Function: | RNF12: E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex- related dosage differences of X-encoded genes in somatic cells of female placental mammals. Belongs to the RNF12 family. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: Xq13-q21 Cellular Component: nucleoplasm; transcriptional repressor complex; cytoplasm; nucleus Molecular Function:zinc ion binding; ubiquitin-protein ligase activity; transcription corepressor activity; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; transcription, DNA-dependent; negative regulation of transcription factor activity; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009] |
| UniProt Code: | Q9NVW2 |
| NCBI GenInfo Identifier: | 143811451 |
| NCBI Gene ID: | 51132 |
| NCBI Accession: | Q9NVW2.3 |
| UniProt Related Accession: | Q9NVW2 |
| Molecular Weight: | Calculated: 52kDa; 68kDaObserved: 75kDa |
| NCBI Full Name: | E3 ubiquitin-protein ligase RLIM |
| NCBI Synonym Full Names: | ring finger protein, LIM domain interacting |
| NCBI Official Symbol: | RLIM |
| NCBI Official Synonym Symbols: | MRX61; RNF12; TOKAS; NY-REN-43 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase RLIM |
| UniProt Protein Name: | E3 ubiquitin-protein ligase RLIM |
| UniProt Synonym Protein Names: | LIM domain-interacting RING finger protein; RING finger LIM domain-binding protein; R-LIM; RING finger protein 12; Renal carcinoma antigen NY-REN-43 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | RLIM |
| UniProt Entry Name: | RNF12_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RLIM antibody (CAB15837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 150s. |
View AllClose