Anti-RIPK3 Antibody (CAB12996)
- SKU:
- CAB12996
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| 抗体名: | Anti-RIPK3 Antibody |
| 抗体コード: | CAB12996 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of mouse RIPK3 |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HT-29, BxPC-3, NIH/3T3, Rat spleen |
| 免疫原: | Recombinant protein of mouse RIPK3 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 56532 |
| Uniprot: | E9PZP3 |
| セルラーロケーション: | Cell membrane, Cytoplasm, Mitochondrion, cytosol |
| 計算された分子量: | 46kDa |
| 観察された分子量: | 50-57kDa |
| 同義語: | RIPK3, RIP3 |
| バックグラウンド: | The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. |
| UniProt Protein Function: | RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; RIPK family Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: mitochondrion; plasma membrane; cytosol Molecular Function:NF-kappaB-inducing kinase activity; identical protein binding; protein serine/threonine kinase activity; protein binding; transcription coactivator activity; protein complex binding; ATP binding; protein kinase activity Biological Process: I-kappaB kinase/NF-kappaB cascade; protein heterooligomerization; protein amino acid autophosphorylation; T cell differentiation in the thymus; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; positive regulation of interferon type I production; T cell homeostasis; positive regulation of ligase activity; toll-like receptor 4 signaling pathway; protein homooligomerization; positive regulation of oxidoreductase activity; spleen development; regulation of activated T cell proliferation; thymus development; MyD88-independent toll-like receptor signaling pathway; protein modification process; lymph node development; regulation of interferon-gamma production; activation of protein kinase activity; toll-like receptor signaling pathway; innate immune response |
| NCBI Summary: | The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008] |
| UniProt Code: | E9PZP3 |
| NCBI GenInfo Identifier: | |
| NCBI Gene ID: | 11035 |
| NCBI Accession: | |
| UniProt Related Accession: | Q9Y572 |
| Molecular Weight: | 518 |
| NCBI Full Name: | |
| NCBI Synonym Full Names: | receptor-interacting serine-threonine kinase 3 |
| NCBI Official Symbol: | RIPK3 |
| NCBI Official Synonym Symbols: | RIP3 |
| NCBI Protein Information: | receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3; receptor interacting protein 3; receptor-interacting protein 3 |
| UniProt Protein Name: | Receptor-interacting serine/threonine-protein kinase 3 |
| UniProt Synonym Protein Names: | Receptor-interacting serine/threonine-protein kinase 3 |
| Protein Family: | Receptor-interacting serine/threonine-protein kinase |
| UniProt Gene Name: | Ripk3 |
| UniProt Entry Name: | E9PZP3_MOUSE |
 | Western blot analysis of extracts of various cell lines, using RIPK3 antibody (CAB12996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Immunofluorescence analysis of Jurkat cells using RIPK3 Rabbit pAb (CAB12996) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of RAW264.7 cells using RIPK3 Rabbit pAb (CAB12996) at dilution of 1:100. Blue: DAPI for nuclear staining. |