Anti-Ribonuclease H1 Antibody (CAB9116)
- SKU:
- CAB9116
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-Ribonuclease H1 Antibody |
| 抗体コード: | CAB9116 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human RNASEH1 (NP_002927.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse testis, Rat thymus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human RNASEH1 (NP_002927.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSWL LFLA HRVA LAAL PCRR GSRG FGMF YAVR RGRK TGVF LTWN ECRA QVDR FPAA RFKK FATE DEAW AFVR KSAS PEVS EGHE NQHG QESE AKAS KRLR EPLD GDGH ESAE PYAK HMKP SVEP APPV SRDT FSYM GDFV VVYT DGCC SSNG RRRP RAGI GVYW GPGH PLNV GIRL PGRQ TNQR AEIH AACK AIEQ AKTQ NINK LVLY TDSM FTIN GITN WVQG WKKN GWKT SAGK EVIN KEDF VALE RLTQ GMDI QWMH VPGH SGFI GNEE ADRL AREG AKQS ED |
| 遺伝子ID: | 246243 |
| Uniprot: | O60930 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 32kDa |
| 観察された分子量: | 37kDa |
| 同義語: | RNASEH1, H1RNA, PEOB2, RNH1 |
| バックグラウンド: | This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which overlaps the first initiation start codon in human. An alternately spliced transcript variant has been found which encodes a shorter isoform. This gene has three pseudogenes; two of them are at different locations of chromosome 17 and one of them is on chromosome 1q32.2. |
| NCBI Summary: | This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are directed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17. [provided by RefSeq, Jul 2017] |
| UniProt Code: | O60930 |
| NCBI GenInfo Identifier: | 21359816 |
| NCBI Gene ID: | 246243 |
| NCBI Accession: | NP_002927 |
| UniProt Related Accession: | O60930 |
| Molecular Weight: | Predicted Molecular Mass: 62.1kDaAccurate Molecular Mass: 62kDa |
| NCBI Full Name: | ribonuclease H1 isoform 1 |
| NCBI Synonym Full Names: | ribonuclease H1 |
| NCBI Official Symbol: | RNASEH1 |
| NCBI Official Synonym Symbols: | RNH1; H1RNA; PEOB2 |
| NCBI Protein Information: | ribonuclease H1 |
| UniProt Protein Name: | Ribonuclease H1 |
| UniProt Synonym Protein Names: | Ribonuclease H type II |
| Protein Family: | Ribonuclease |
| UniProt Gene Name: | RNASEH1 |
| UniProt Entry Name: | RNH1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RNASEH1 antibody (CAB9116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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