Anti-RhoGDI[KO Validated] Antibody (CAB11556)
![Anti-RhoGDI[KO Validated] Antibody (CAB11556)](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/16753/16629/anti-rhogdiko-validated-antibody-cab11556__11147__34290.1645803554.jpg?c=1 "Anti-RhoGDI[KO Validated] Antibody (CAB11556)")
- SKU:
- CAB11556
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Cell Biology
![Anti-RhoGDI[KO Validated] Antibody (CAB11556)](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/16753/16629/anti-rhogdiko-validated-antibody-cab11556__11147__34290.1645803554.jpg?c=1&imbypass=on "Anti-RhoGDI[KO Validated] Antibody (CAB11556)")
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Description
| 抗体名: | Anti-RhoGDI[KO Validated] Antibody |
| 抗体コード: | CAB11556 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthesized peptide derived from human RhoGDI |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, 293T, NIH/3T3, U-251MG, Mouse brain, Rat liver |
| 免疫原: | A synthesized peptide derived from human RhoGDI |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 396 |
| Uniprot: | P52565 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 26kDa |
| 観察された分子量: | 26KDa |
![Anti-RhoGDI[KO Validated] Antibody](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/w/800/A11556_1__04033.jpg) | Western blot analysis of extracts of various cell lines, using RhoGDI Rabbit mAb (CAB11556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
![Anti-RhoGDI[KO Validated] Antibody](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/b/396/A11556_2__64068.jpg) | Western blot analysis of extracts from normal (control) and RhoGDI knockout (KO) 293T cells, using RhoGDI antibody (CAB11556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
| 同義語: | GDIA1, HEL-S-47e, NPHS8, RHOGDI, RHOGDI-1 |
| バックグラウンド: | This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Protein Function: | RhoGDI alpha: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Monomer. Forms a heterodimer with RAC1. Interacts with FER. Belongs to the Rho GDI family. |
| UniProt Protein Details: | Protein type:Apoptosis; Cell development/differentiation; G protein regulator, misc.; Cell adhesion; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: cytoskeleton; cytosol Molecular Function:GTPase activator activity; protein binding; Rho GDP-dissociation inhibitor activity Biological Process: cell motility; negative regulation of apoptosis; negative regulation of axonogenesis; negative regulation of cell adhesion; nerve growth factor receptor signaling pathway; positive regulation of axonogenesis; positive regulation of GTPase activity; regulation of axonogenesis; regulation of catalytic activity; regulation of small GTPase mediated signal transduction; Rho protein signal transduction; small GTPase mediated signal transduction Disease: Nephrotic Syndrome, Type 8 |
| NCBI Summary: | This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | P52565 |
| NCBI GenInfo Identifier: | 1707892 |
| NCBI Gene ID: | 396 |
| NCBI Accession: | P52565.3 |
| UniProt Secondary Accession: | P52565,Q6IBM5, A8MXW0, B2R5X1, B4DDD3, B4DUV9, |
| UniProt Related Accession: | P52565 |
| Molecular Weight: | 18,233 Da |
| NCBI Full Name: | Rho GDP-dissociation inhibitor 1 |
| NCBI Synonym Full Names: | Rho GDP dissociation inhibitor alpha |
| NCBI Official Symbol: | ARHGDIA |
| NCBI Official Synonym Symbols: | GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e |
| NCBI Protein Information: | rho GDP-dissociation inhibitor 1 |
| UniProt Protein Name: | Rho GDP-dissociation inhibitor 1 |
| UniProt Synonym Protein Names: | Rho-GDI alpha |
| UniProt Gene Name: | ARHGDIA |
| UniProt Entry Name: | GDIR1_HUMAN |