Anti-RHOC Antibody (CAB1062)[KO Validated]

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SKU:
CAB1062
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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Description

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抗体名:Anti-RHOC Antibody [KO Validated]
抗体コード:CAB1062
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC IF
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1).
申し込み:WB IHC IF
推奨希釈:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
反応性:Human, Mouse, Rat
ポジティブサンプル:U2OS, Jurkat, A-549, PC-12
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MAAI RKKL VIVG DGAC GKTC LLIV FSKD QFPE VYVP TVFE NYIA DIEV DGKQ VELA LWDT AGQE DYDR LRPL SYPD TDVI LMCF SIDS PDSL ENIP EKWT PEVK HFCP NVPI ILVG NKKD LRQD EHTR RELA KMKQ EPVR SEEG RDMA NRIS AFGY LECS AKTK EGVR EVFE MATR AGLQ VRKN KRRR GCPI L
遺伝子ID:389
Uniprot:P08134
セルラーロケーション:Cell membrane, Cleavage furrow, Cytoplasmic side, Lipid-anchor
計算された分子量:22kDa
観察された分子量:22kDa
同義語:RHOC, ARH9, ARHC, H9, RHOH9
バックグラウンド:This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
UniProt Protein Function:RHOC: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.
UniProt Protein Details:

Protein type:G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, Rho; G protein

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: cleavage furrow; cytosol; plasma membrane

Molecular Function:protein binding; signal transducer activity

Biological Process: apical junction assembly; cytokinesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of lipase activity; regulation of small GTPase mediated signal transduction

NCBI Summary:This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:P08134
NCBI GenInfo Identifier:132543
NCBI Gene ID:389
NCBI Accession:P08134.1
UniProt Secondary Accession:P08134,Q6ICN3, B3KSW1,
UniProt Related Accession:P08134
Molecular Weight:22,006 Da
NCBI Full Name:Rho-related GTP-binding protein RhoC
NCBI Synonym Full Names:ras homolog family member C
NCBI Official Symbol:RHOC  
NCBI Official Synonym Symbols:H9; ARH9; ARHC; RHOH9  
NCBI Protein Information:rho-related GTP-binding protein RhoC
UniProt Protein Name:Rho-related GTP-binding protein RhoC
UniProt Synonym Protein Names:Rho cDNA clone 9; h9
Protein Family:Rho-related GTP-binding protein
UniProt Gene Name:RHOC  
UniProt Entry Name:RHOC_HUMAN
Anti-RHOC Antibody [KO Validated] (CAB1062)Western blot analysis of extracts of various cell lines, using RHOC antibody (CAB1062). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-RHOC Antibody [KO Validated] (CAB1062)Western blot analysis of extracts from normal (control) and RHOC knockout (KO) 293T cells, using RHOC antibody (CAB1062) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 300s.
Anti-RHOC Antibody [KO Validated] (CAB1062)Immunofluorescence analysis of HeLa cells using RHOC antibody (CAB1062). Blue: DAPI for nuclear staining.
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