Anti-RCN1 Antibody (CAB10572)
- SKU:
- CAB10572
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-RCN1 Antibody |
| 抗体コード: | CAB10572 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-331 of human RCN1 (NP_002892.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:1000 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | A-549, SKOV3, LO2, HeLa |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-331 of human RCN1 (NP_002892.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | KPTV RKER VVRP DSEL GERP PEDN QSFQ YDHE AFLG KEDS KTFD QLTP DESK ERLG KIVD RIDN DGDG FVTT EELK TWIK RVQK RYIF DNVA KVWK DYDR DKDD KISW EEYK QATY GYYL GNPA EFHD SSDH HTFK KMLP RDER RFKA ADLN GDLT ATRE EFTA FLHP EEFE HMKE IVVL ETLE DIDK NGDG FVDQ DEYI ADMF SHEE NGPE PDWV LSER EQFN EFRD LNKD GKLD KDEI RHWI LPQD YDHA QAEA RHLV YESD KNKD EKLT KEEI LENW NMFV GSQA TNYG EDLT KNHD EL |
| 遺伝子ID: | 5954 |
| Uniprot: | Q15293 |
| セルラーロケーション: | Endoplasmic reticulum lumen |
| 計算された分子量: | 33kDa/38kDa |
| 観察された分子量: | 45kDa |
| 同義語: | RCN1, HEL-S-84, PIG20, RCAL, RCN |
| バックグラウンド: | Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane. |
| UniProt Protein Function: | RCN1: May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Belongs to the CREC family. |
| UniProt Protein Details: | Protein type:Calcium-binding Chromosomal Location of Human Ortholog: 11p13 Cellular Component: endoplasmic reticulum Molecular Function:protein binding |
| NCBI Summary: | Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.[provided by RefSeq, Jan 2009] |
| UniProt Code: | Q15293 |
| NCBI GenInfo Identifier: | 2493462 |
| NCBI Gene ID: | 5954 |
| NCBI Accession: | Q15293.1 |
| UniProt Secondary Accession: | Q15293,B7Z1M1, D3DR00, |
| UniProt Related Accession: | Q15293 |
| Molecular Weight: | 39kDa |
| NCBI Full Name: | Reticulocalbin-1 |
| NCBI Synonym Full Names: | reticulocalbin 1 |
| NCBI Official Symbol: | RCN1 |
| NCBI Official Synonym Symbols: | RCN; RCAL; PIG20; HEL-S-84 |
| NCBI Protein Information: | reticulocalbin-1 |
| UniProt Protein Name: | Reticulocalbin-1 |
| Protein Family: | Reticulocalbin |
| UniProt Gene Name: | RCN1 |
 | Western blot analysis of extracts of various cell lines, using RCN1 antibody (CAB10572) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of L929 cells using RCN1 Rabbit pAb (CAB10572) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using RCN1 Rabbit pAb (CAB10572) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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