Anti-RBBP8 Antibody (CAB10201)
- SKU:
- CAB10201
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-RBBP8 Antibody |
| 抗体コード: | CAB10201 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RBBP8 (NP_976036.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, HT-29, Mouse spleen, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RBBP8 (NP_976036.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MNIS GSSC GSPN SADT SSDF KDLW TKLK ECHD REVQ GLQV KVTK LKQE RILD AQRL EEFF TKNQ QLRE QQKV LHET IKVL EDRL RAGL CDRC AVTE EHMR KKQQ EFEN IRQQ NLKL ITEL MNER NTLQ EENK KLSE QLQQ KIEN DQQH QAAE LECE EDVI PDSP ITAF SFSG VNRL RRKE NPHV RYIE QTHT KLEH SVCA NEMR KVSK SSTH PQHN PNEN EILV ADTY DQSQ SPMA KAHG TSSY TPDK SSFN LATV VAET LGLG VQEE SETQ GPMS PLGD ELYH CLEG NHKK QPFE ESTR |
| 遺伝子ID: | 5932 |
| Uniprot: | Q99708 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 98kDa/101kDa/102kDa |
| 観察された分子量: | 110kDa |
| 同義語: | RBBP8, COM1, CTIP, JWDS, RIM, SAE2, SCKL2 |
| バックグラウンド: | The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. |
| UniProt Protein Function: | CtIP: a transcription factor containing C2H2 zinc fingers. May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. Interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein. Abundantly expressed in brain and the immune system and associated with immune system malignancies. Ionizing radiation induces its hyperphosphorylation and dissociation from BRCA1 in an ATM-dependent manner. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; EC 3.1.-.- Chromosomal Location of Human Ortholog: 18q11.2 Cellular Component: nucleolus; nucleoplasm; nucleus; transcriptional repressor complex Molecular Function:damaged DNA binding; protein binding; single-stranded DNA specific endodeoxyribonuclease activity Biological Process: cell cycle checkpoint; DNA double-strand break processing; DNA repair; DNA replication; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; G2/M transition DNA damage checkpoint; nucleotide-excision repair; regulation of transcription from RNA polymerase II promoter; strand displacement Disease: Jawad Syndrome; Seckel Syndrome 2 |
| NCBI Summary: | The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99708 |
| NCBI GenInfo Identifier: | 116242745 |
| NCBI Gene ID: | 5932 |
| NCBI Accession: | Q99708.2 |
| UniProt Secondary Accession: | Q99708,O75371, Q8NHQ3, A6NKN2, A8K8W6, E7ETY1, |
| UniProt Related Accession: | Q99708 |
| Molecular Weight: | 98,434 Da |
| NCBI Full Name: | DNA endonuclease RBBP8 |
| NCBI Synonym Full Names: | RB binding protein 8, endonuclease |
| NCBI Official Symbol: | RBBP8 |
| NCBI Official Synonym Symbols: | RIM; COM1; CTIP; JWDS; SAE2; SCKL2 |
| NCBI Protein Information: | DNA endonuclease RBBP8 |
| UniProt Protein Name: | DNA endonuclease RBBP8 |
| UniProt Synonym Protein Names: | CtBP-interacting protein; CtIP; Retinoblastoma-binding protein 8; RBBP-8; Retinoblastoma-interacting protein and myosin-like; RIM; Sporulation in the absence of SPO11 protein 2 homolog; SAE2 |
| Protein Family: | RBBP8 N-terminal-like protein |
| UniProt Gene Name: | RBBP8 |
| UniProt Entry Name: | COM1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RBBP8 antibody (CAB10201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of extracts of various cell lines, using RBBP8 antibody (CAB10201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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