Anti-RAPGEF3 Antibody (CAB2199)
- SKU:
- CAB2199
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cardiovascular
Description
| 抗体名: | Anti-RAPGEF3 Antibody |
| 抗体コード: | CAB2199 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RAPGEF3 (NP_001092002.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Mouse heart, Mouse thymus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RAPGEF3 (NP_001092002.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MVLR RMHR PRSC SYQL LLEH QRPS CIQG LRWT PLTN SEES LDFS ESLE QAST ERVL RAGR QLHR HLLA TCPN LIRD RKYH LRLY RQCC SGRE LVDG ILAL GLGV HSRS QVVG ICQV LLDE GALC HVKH DWAF QDRD AQFY RFPG PEPE PVGT HEME EELA EAVA LLSQ RGPD ALLT VALR KPPG QRTD EELD LIFE ELLH |
| 遺伝子ID: | 10411 |
| Uniprot: | O95398 |
| セルラーロケーション: | Endomembrane system |
| 計算された分子量: | 66kDa/99kDa/103kDa |
| 観察された分子量: | 104kDa |
| 同義語: | RAPGEF3, CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910 |
| バックグラウンド: |
| UniProt Protein Function: | Epac1: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GEFs, misc.; GEFs Chromosomal Location of Human Ortholog: 12q13.1 Cellular Component: membrane; plasma membrane; endomembrane system; cAMP-dependent protein kinase complex Molecular Function:Rap guanyl-nucleotide exchange factor activity; protein binding; guanyl-nucleotide exchange factor activity; cAMP-dependent protein kinase regulator activity; cAMP binding Biological Process: positive regulation of syncytium formation by plasma membrane fusion; platelet activation; signal transduction; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein export from nucleus; cell proliferation; positive regulation of angiogenesis; cAMP-mediated signaling; positive regulation of stress fiber formation; energy reserve metabolic process; positive regulation of cAMP catabolic process; angiogenesis; blood coagulation; regulation of protein kinase activity; regulation of insulin secretion; Rap protein signal transduction |
| UniProt Code: | O95398 |
| NCBI GenInfo Identifier: | 317373593 |
| NCBI Gene ID: | 10411 |
| NCBI Accession: | O95398.6 |
| UniProt Related Accession: | O95398 |
| Molecular Weight: | |
| NCBI Full Name: | Rap guanine nucleotide exchange factor 3 |
| NCBI Synonym Full Names: | Rap guanine nucleotide exchange factor 3 |
| NCBI Official Symbol: | RAPGEF3 |
| NCBI Official Synonym Symbols: | EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI |
| NCBI Protein Information: | rap guanine nucleotide exchange factor 3 |
| UniProt Protein Name: | Rap guanine nucleotide exchange factor 3 |
| UniProt Synonym Protein Names: | Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP; cAMP-regulated guanine nucleotide exchange factor I |
| UniProt Gene Name: | RAPGEF3 |
| UniProt Entry Name: | RPGF3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RAPGEF3 antibody (CAB2199) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of U2OS cells using RAPGEF3 antibody (CAB2199). Blue: DAPI for nuclear staining. |