Anti-RALA Antibody (CAB0541)
- SKU:
- CAB0541
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-RALA Antibody |
| 抗体コード: | CAB0541 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human RALA (NP_005393.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse lung, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human RALA (NP_005393.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAAN KPKG QNSL ALHK VIMV GSGG VGKS ALTL QFMY DEFV EDYE PTKA DSYR KKVV LDGE EVQI DILD TAGQ EDYA AIRD NYFR SGEG FLCV FSIT EMES FAAT ADFR EQIL RVKE DENV PFLL VGNK SDLE DKRQ VSVE EAKN RAEQ WNVN YVET SAKT RANV DKVF FDLM REIR ARKM EDSK EKNG KKKR KSLA KRIR ERCC IL |
| 遺伝子ID: | 5898 |
| Uniprot: | P11233 |
| セルラーロケーション: | Cell membrane, Cell surface, Cleavage furrow, Cytoplasmic side, Lipid-anchor, Midbody |
| 計算された分子量: | 23kDa |
| 観察された分子量: | 24kDa |
| 同義語: | RALA, RAL |
| バックグラウンド: | The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. |
| UniProt Protein Function: | RALA: Multifuntional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin- dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells. Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. Interacts with LPAR1 and LPAR2. Interacts with ADRBK1 in response to LPAR1 activation. RALA and ADRBK1 mutually inhibit each other's binding to LPAR1. Activated in an LPA-dependent manner by LPAR1 and in an LPA-independent manner by LPAR2. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; G protein, monomeric, Ras; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 7p15-p13 Cellular Component: cytoplasmic vesicle membrane; cell surface; focal adhesion; endocytic vesicle; plasma membrane; midbody; cleavage furrow Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; myosin binding; Edg-2 lysophosphatidic acid receptor binding; ubiquitin protein ligase binding; ATPase binding Biological Process: positive regulation of filopodium formation; regulation of exocytosis; exocytosis; lipid raft localization; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; Ras protein signal transduction; actin cytoskeleton reorganization; cytokinesis; chemotaxis; signal transduction |
| NCBI Summary: | The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P11233 |
| NCBI GenInfo Identifier: | 131834 |
| NCBI Gene ID: | 5898 |
| NCBI Accession: | P11233.1 |
| UniProt Secondary Accession: | P11233,A4D1W3, |
| UniProt Related Accession: | P11233 |
| Molecular Weight: | 206 |
| NCBI Full Name: | Ras-related protein Ral-A |
| NCBI Synonym Full Names: | v-ral simian leukemia viral oncogene homolog A (ras related) |
| NCBI Official Symbol: | RALA |
| NCBI Official Synonym Symbols: | RAL |
| NCBI Protein Information: | ras-related protein Ral-A; RAS-like protein A; ras related GTP binding protein A; Ras family small GTP binding protein RALA |
| UniProt Protein Name: | Ras-related protein Ral-A |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RALA |
| UniProt Entry Name: | RALA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RALA antibody (CAB0541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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