Anti-RAC3 Antibody (CAB7498)
- SKU:
- CAB7498
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-RAC3 Antibody |
| 抗体コード: | CAB7498 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RAC3 (NP_005043.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | BT-474, HL-60, Human placenta, Mouse lung, Mouse brain, Mouse thymus |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RAC3 (NP_005043.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MQAI KCVV VGDG AVGK TCLL ISYT TNAF PGEY IPTV FDNY SANV MVDG KPVN LGLW DTAG QEDY DRLR PLSY PQTD VFLI CFSL VSPA SFEN VRAK WYPE VRHH CPHT PILL VGTK LDLR DDKD TIER LRDK KLAP ITYP QGLA MARE IGSV KYLE CSAL TQRG LKTV FDEA IRAV LCPP PVKK PGKK CTVF |
| 遺伝子ID: | 5881 |
| Uniprot: | P60763 |
| セルラーロケーション: | Cell membrane, Cell projection, Cytoplasm, Endomembrane system, cytoskeleton, lamellipodium, perinuclear region |
| 計算された分子量: | 21kDa |
| 観察された分子量: | 21kDa |
| 同義語: | RAC3 |
| バックグラウンド: | The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | RAC3: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as cell spreading and the formation of actin-based protusions including lamellipodia and membrane ruffles. Interacts with the GEF protein DOCK7, which promotes the exchange between GDP and GTP, and therefore activates it. Interacts with C1D. Expression down-regulated in quiescent fibroblasts and clearly induced by serum stimulation. Highest levels in brain, also detected in heart, placenta and pancreas. Belongs to the small GTPase superfamily. Rho family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; G protein; G protein, monomeric; G protein, monomeric, Rho Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: filamentous actin; neuron projection; growth cone; cell soma; perinuclear region of cytoplasm; lamellipodium; plasma membrane; endomembrane system; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; calcium-dependent protein binding Biological Process: regulation of small GTPase mediated signal transduction; metabolic process; small GTPase mediated signal transduction; positive regulation of cell adhesion mediated by integrin; cell projection biogenesis; actin cytoskeleton organization and biogenesis; neuromuscular process controlling balance; neurite development |
| NCBI Summary: | The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] |
| UniProt Code: | P60763 |
| NCBI GenInfo Identifier: | 46397673 |
| NCBI Gene ID: | 5881 |
| NCBI Accession: | P60763.1 |
| UniProt Related Accession: | P60763 |
| Molecular Weight: | |
| NCBI Full Name: | Ras-related C3 botulinum toxin substrate 3 |
| NCBI Synonym Full Names: | Rac family small GTPase 3 |
| NCBI Official Symbol: | RAC3 |
| NCBI Protein Information: | ras-related C3 botulinum toxin substrate 3 |
| UniProt Protein Name: | Ras-related C3 botulinum toxin substrate 3 |
| UniProt Synonym Protein Names: | p21-Rac3 |
| Protein Family: | Rac-like GTP-binding protein |
| UniProt Gene Name: | RAC3 |
| UniProt Entry Name: | RAC3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RAC3 antibody (CAB7498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunofluorescence analysis of A549 cells using RAC3 antibody (CAB7498). Blue: DAPI for nuclear staining. |
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