Anti-Rabbit anti GST-Tag Polyclonal Antibody (CABE006)
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- SKU:
- CABE006
- Product type:
- Antibody
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Frequently bought together:
Description
| 抗体名: | Anti-Rabbit anti GST-Tag Polyclonal Antibody |
| 抗体コード: | CABE006 |
| 抗体サイズ: | 100 uL |
| 申し込み: | WB IF IP |
| 反応性: | |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein. |
| 申し込み: | WB IF IP |
| 推奨希釈: | WB 1:1000 - 1:2000 IF 1:20 - 1:50 IP 1:20 - 1:50 |
| 反応性: | |
| ポジティブサンプル: | GST fusion protein |
| 免疫原: | Recombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein. |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSPI LGYW KIKG LVQP TRLL LEYL EEKY EEHL YERD EGDK WRNK KFEL GLEF PNLP YYID GDVK LTQS MAII RYIA DKHN MLGG CPKE RAEI SMLE GAVL DIRY GVSR IAYS KDFE TLKV DFLS KLPE MLKM FEDR LCHK TYLN GDHV THPD FMLY DALD VVLY MDPM CLDA FPKL VCFK KRIE AIPQ IDKY LKSS KYIA WPLQ GWQA TFGG GDHP PK |
| 遺伝子ID: | |
| Uniprot: | |
| セルラーロケーション: | |
| 計算された分子量: | 26kDa |
| 観察された分子量: | Refer to Figures |
 | Western blot analysis of over-expressed GST protein using GST-tag antibody (CABE006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunoprecipitation analysis of 50ug extracts of GST protein cells using 3ug Rabbit anti GST-Tag pAb antibody (CABE006). Western blot was performed from the immunoprecipitate using Rabbit anti GST-Tag pAb antibody (CABE006) at a dilition of 1:20000. |
| 同義語: | GST, GST tag, GST-tag |
| バックグラウンド: | Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal€”also known as MAPEG€”proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. |