Anti-RAB6A Antibody (CAB5613)
- SKU:
- CAB5613
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-RAB6A Antibody |
| 抗体コード: | CAB5613 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RAB6A (NP_002860.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | BT-474, OVCAR3, SW480, HT-29, 293T, SH-SY5Y, Mouse brain, Mouse lung, Mouse intestine |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RAB6A (NP_002860.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MSTG GDFG NPLR KFKL VFLG EQSV GKTS LITR FMYD SFDN TYQA TIGI DFLS KTMY LEDR TIRL QLWD TAGQ ERFR SLIP SYIR DSAA AVVV YDIT NVNS FQQT TKWI DDVR TERG SDVI IMLV GNKT DLAD KRQV SIEE GERK AKEL NVMF IETS AKAG YNVK QLFR RVAA ALPG MEST QDRS REDM IDIK LEKP QEQP |
| 遺伝子ID: | 5870 |
| Uniprot: | P20340 |
| セルラーロケーション: | Golgi apparatus, Golgi apparatus membrane, Lipid-anchor |
| 計算された分子量: | 11kDa/19kDa/23kDa |
| 観察された分子量: | 33kDa |
| 同義語: | RAB6A, RAB6 |
| バックグラウンド: | This gene encodes a member of the RAB family, which belongs to the small GTPase superfamily. GTPases of the RAB family bind to various effectors to regulate the targeting and fusion of transport carriers to acceptor compartments. This protein is located at the Golgi apparatus, which regulates trafficking in both a retrograde (from early endosomes and Golgi to the endoplasmic reticulum) and an anterograde (from the Golgi to the plasma membrane) directions. Myosin II is an effector of this protein in these processes. This protein is also involved in assembly of human cytomegalovirus (HCMV) by interacting with the cellular protein Bicaudal D1, which interacts with the HCMV virion tegument protein, pp150. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | RAB6A: Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity. Interacts with CCDC64; leads to its accumulation in the pericentrosomal region. Interacts with SCYL1BP1. Interacts with VSP52 and RABGAP1. Interacts with GCC2 (via its GRIP domain). Interacts with RAB6IP1 (via its RUN 1 domain). Isoform 1 interacts with RAB6KIFL. Isoform 2 does not interact with RAB6KIFL. Isoform 1 interacts with BICD1. Isoform 2 interacts with BICD1. Isoform 1 interacts with BICD2. Isoform 2 interacts with BICD2. Interacts with TMF1. Isoform 1 (GTP-bound) interacts with DYNLRB1; the interaction is direct. Isoform 2 (GDP-bound) interacts with DYNLRB1; the interaction is direct. Interacts with PIFO/C1orf88. Ubiquitous. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:G protein, monomeric, Rab; G protein, monomeric; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11q13.3 Cellular Component: cytoplasmic vesicle; cytosol; Golgi apparatus; Golgi membrane; membrane; trans-Golgi network Molecular Function:GTP binding; GTPase activity; myosin V binding; protein binding; protein domain specific binding Biological Process: antigen processing and presentation; peptidyl-cysteine methylation; protein localization in Golgi apparatus; protein targeting to Golgi; retrograde vesicle-mediated transport, Golgi to ER; small GTPase mediated signal transduction; viral reproduction |
| NCBI Summary: | This gene encodes a member of the RAB family, which belongs to the small GTPase superfamily. GTPases of the RAB family bind to various effectors to regulate the targeting and fusion of transport carriers to acceptor compartments. This protein is located at the Golgi apparatus, which regulates trafficking in both a retrograde (from early endosomes and Golgi to the endoplasmic reticulum) and an anterograde (from the Golgi to the plasma membrane) directions. Myosin II is an effector of this protein in these processes. This protein is also involved in assembly of human cytomegalovirus (HCMV) by interacting with the cellular protein Bicaudal D1, which interacts with the HCMV virion tegument protein, pp150. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P20340 |
| NCBI GenInfo Identifier: | 131796 |
| NCBI Gene ID: | 5870 |
| NCBI Accession: | P20340.3 |
| UniProt Secondary Accession: | P20340,Q1W5D8, Q5U0A8, Q9UBE4, A8K133, B7Z772, F5H668 |
| UniProt Related Accession: | P20340 |
| Molecular Weight: | 19,935 Da |
| NCBI Full Name: | Ras-related protein Rab-6A |
| NCBI Synonym Full Names: | RAB6A, member RAS oncogene family |
| NCBI Official Symbol: | RAB6A |
| NCBI Official Synonym Symbols: | RAB6 |
| NCBI Protein Information: | ras-related protein Rab-6A |
| UniProt Protein Name: | Ras-related protein Rab-6A |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAB6A |
| UniProt Entry Name: | RAB6A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RAB6A antibody (CAB5613) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
 | Immunofluorescence analysis of HeLa cells using RAB6A antibody (CAB5613). Blue: DAPI for nuclear staining. |
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