Anti-RAB35 Antibody (CAB8030)
- SKU:
- CAB8030
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | RAB35 Rabbit Polyclonal Antibody |
| 抗体コード: | CAB8030 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human RAB35 (NP_006852.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | BT-474, Mouse brain, Mouse lung, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human RAB35 (NP_006852.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MARD YDHL FKLL IIGD SGVG KSSL LLRF ADNT FSGS YITT IGVD FKIR TVEI NGEK VKLQ IWDT AGQE RFRT ITST YYRG THGV IVVY DVTS AESF VNVK RWLH EINQ NCDD VCRI LVGN KNDD PERK VVET EDAY KFAG QMGI QLFE TSAK ENVN VEEM FNCI TELV LRAK KDNL AKQQ QQQQ NDVV KLTK NSKR KKRC C |
| 遺伝子ID: | 11021 |
| Uniprot: | Q15286 |
| セルラーロケーション: | Cell membrane, Cytoplasmic side, Cytoplasmic vesicle, Endosome, Lipid-anchor, Melanosome, Membrane, clathrin-coated pit, clathrin-coated vesicle |
| 計算された分子量: | 17kDa/23kDa |
| 観察された分子量: | 34kDa |
| 同義語: | RAB35, H-ray, RAB1C, RAY |
| バックグラウンド: |
| UniProt Protein Function: | RAB35: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. Interacts with DENND1A and DENND1B in a nucleotide- dependent manner. The low binding to DENND1C is nucleotide- independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:G protein; G protein, monomeric, Rab; G protein, monomeric Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: cell projection membrane; clathrin-coated endocytic vesicle; coated pit; endoplasmic reticulum membrane; endosome membrane; Golgi membrane; intercellular bridge; plasma membrane Molecular Function:GDP binding; GTP binding; GTPase activity; phosphatidylinositol-4,5-bisphosphate binding; protein binding Biological Process: antigen processing and presentation; cytokinesis; endocytic recycling; endosome transport; ER to Golgi vesicle-mediated transport; neurite development; plasma membrane to endosome transport; protein localization |
| UniProt Code: | Q15286 |
| NCBI GenInfo Identifier: | 3024525 |
| NCBI Gene ID: | 11021 |
| NCBI Accession: | Q15286.1 |
| UniProt Secondary Accession: | Q15286,B2R6E0, B4E390, |
| UniProt Related Accession: | Q15286 |
| Molecular Weight: | 17,058 Da |
| NCBI Full Name: | Ras-related protein Rab-35 |
| NCBI Synonym Full Names: | RAB35, member RAS oncogene family |
| NCBI Official Symbol: | RAB35 |
| NCBI Official Synonym Symbols: | RAY; H-ray; RAB1C |
| NCBI Protein Information: | ras-related protein Rab-35 |
| UniProt Protein Name: | Ras-related protein Rab-35 |
| UniProt Synonym Protein Names: | GTP-binding protein RAY; Ras-related protein Rab-1C |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAB35 |
| UniProt Entry Name: | RAB35_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RAB35 antibody (CAB8030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
View AllClose