Anti-PTPN22 抗体 (CAB1406)

抗体名:	Anti-PTPN22 Antibody
抗体コード:	CAB1406
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human PTPN22 (NP_036543.4).

申し込み:	WB IF
推奨希釈:	WB 1:500 - 1:2000 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	Jurkat, Mouse spleen

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human PTPN22 (NP_036543.4).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	MDQR EILQ KFLD EAQS KKIT KEEF ANEF LKLK RQST KYKA DKTY PTTV AEKP KNIK KNRY KDIL PYDY SRVE LSLI TSDE DSSY INAN FI
遺伝子ID:	26191
Uniprot:	Q9Y2R2
セルラーロケーション:	Cytoplasm
計算された分子量:	20kDa/75kDa/78kDa/85kDa/88kDa/91kDa
観察された分子量:	107kDa

同義語:

PTPN22, LYP, LYP1, LYP2, PEP, PTPN22.5, PTPN22.6, PTPN8

バックグラウンド:

This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described.

UniProt Protein Function:	PTPN22: Seems to act on Cbl. May play a role in regulating the function of Cbl and its associated protein kinases. Acts as negative regulator of T-cell receptor (TCR) signaling. Dephosphorylates and inactivates the SRC family kinases. Interacts with CSK. Interacts with LPXN. Predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells. Down-regulated by phosphorylation. Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis; EC 3.1.3.48 Chromosomal Location of Human Ortholog: 1p13.2 Cellular Component: cytoplasm; internal side of plasma membrane; nucleus; perinuclear region of cytoplasm Molecular Function:kinase binding; phosphoric monoester hydrolase activity; protein binding; protein tyrosine phosphatase activity; SH3 domain binding; ubiquitin protein ligase binding Biological Process: lipopolysaccharide-mediated signaling pathway; negative regulation of autophagy; negative regulation of JNK activity; negative regulation of T cell activation; negative regulation of T cell receptor signaling pathway; negative regulation of tumor necrosis factor production; positive regulation of interferon type I production; positive regulation of toll-like receptor 3 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway; protein amino acid dephosphorylation; regulation of innate immune response; regulation of natural killer cell proliferation; response to lipopolysaccharide; T cell differentiation Disease: Diabetes Mellitus, Insulin-dependent; Rheumatoid Arthritis; Systemic Lupus Erythematosus
NCBI Summary:	This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009]
UniProt Code:	Q9Y2R2
NCBI GenInfo Identifier:	20139861
NCBI Gene ID:	26191
NCBI Accession:	Q9Y2R2.2
UniProt Secondary Accession:	Q9Y2R2,O95063, O95064, Q6IPX8, Q8WVM1, A0N0K6, B1ALC8 D4NZ71, E9PLD8, E9PPI1,
UniProt Related Accession:	Q9Y2R2
Molecular Weight:	75,488 Da
NCBI Full Name:	Tyrosine-protein phosphatase non-receptor type 22
NCBI Synonym Full Names:	protein tyrosine phosphatase, non-receptor type 22
NCBI Official Symbol:	PTPN22
NCBI Official Synonym Symbols:	LYP; PEP; LYP1; LYP2; PTPN8
NCBI Protein Information:	tyrosine-protein phosphatase non-receptor type 22
UniProt Protein Name:	Tyrosine-protein phosphatase non-receptor type 22
UniProt Synonym Protein Names:	Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; LyP; PEST-domain phosphatase; PEP
Protein Family:	Tyrosine-protein phosphatase non-receptor
UniProt Gene Name:	PTPN22
UniProt Entry Name:	PTN22_HUMAN

	Western blot analysis of extracts of various cell lines, using PTPN22 antibody (CAB1406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of C6 cells using PTPN22 antibody (CAB1406) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L-929 cells using PTPN22 antibody (CAB1406) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using PTPN22 antibody (CAB1406) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-PTPN22 Antibody (CAB17128)

PTPN22 Antibody (PACO03823)

PTPN22 Antibody (PACO11590)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)