Anti-PTPN11 Antibody (RACO0257)
- SKU:
- RACO0257
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
| 商品名: | PTPN11 Antibody |
| Product SKU: | RACO0257 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IHC |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human SHP2 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73 (PubMed:26742426). |
| シノニム: | Tyrosine-protein phosphatase non-receptor type 11 (EC 3.1.3.48) (Protein-tyrosine phosphatase 1D) (PTP-1D) (Protein-tyrosine phosphatase 2C) (PTP-2C) (SH-PTP2) (SHP-2) (Shp2) (SH-PTP3), PTPN11, PTP2C SHPTP2 |
 | IHC image of RACO0257 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
 | Western Blot. Positive WB detected in Jurkat whole cell lysate 293 whole cell lysate MCF-7 whole cell lysate Hela whole cell lysate K562 whole cell lysate HL60 whole cell lysate All lanes PTPN11 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 69, 53 kDa. Observed band size: 69 kDa. |
| UniProt Protein Function: | SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48 Chromosomal Location of Human Ortholog: 12q24 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:1-phosphatidylinositol-3-kinase activity; insulin receptor binding; non-membrane spanning protein tyrosine phosphatase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphoprotein phosphatase activity; protein binding; protein tyrosine phosphatase activity; SH3/SH2 adaptor activity Biological Process: brain development; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; genitalia development; heart development; inner ear development; leukocyte migration; phosphoinositide-mediated signaling; platelet activation; regulation of cell adhesion mediated by integrin; regulation of phosphoinositide 3-kinase cascade; regulation of protein complex assembly; T cell costimulation Disease: Juvenile Myelomonocytic Leukemia; Leopard Syndrome 1; Metachondromatosis; Noonan Syndrome 1 |
| NCBI Summary: | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | Q06124 |
| NCBI GenInfo Identifier: | 84028248 |
| NCBI Gene ID: | 5781 |
| NCBI Accession: | Q06124.2 |
| UniProt Secondary Accession: | Q06124,Q96HD7, A8K1D9, |
| UniProt Related Accession: | Q06124 |
| Molecular Weight: | 52,828 Da |
| NCBI Full Name: | Tyrosine-protein phosphatase non-receptor type 11 |
| NCBI Synonym Full Names: | protein tyrosine phosphatase, non-receptor type 11 |
| NCBI Official Symbol: | PTPN11 |
| NCBI Official Synonym Symbols: | CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3 |
| NCBI Protein Information: | tyrosine-protein phosphatase non-receptor type 11 |
| UniProt Protein Name: | Tyrosine-protein phosphatase non-receptor type 11 |
| UniProt Synonym Protein Names: | Protein-tyrosine phosphatase 1D; PTP-1D; Protein-tyrosine phosphatase 2C; PTP-2C; SH-PTP2; SHP-2; Shp2; SH-PTP3 |
| Protein Family: | Tyrosine-protein phosphatase non-receptor |
| UniProt Gene Name: | PTPN11 |
| UniProt Entry Name: | PTN11_HUMAN |