Anti-PTGS2 Antibody (RACO0524)
- SKU:
- RACO0524
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Conjugation:
- Unconjugated
Description
| 商品名: | PTGS2 Antibody |
| Product SKU: | RACO0524 |
| サイズ: | 50ul |
| 宿主種: | Homo sapiens (Human) |
| 申し込み: | ELISA, WB, IF |
| 推奨される希釈: | WB:1:500-1:5000, IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human COX2 |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. |
| シノニム: | Prostaglandin G/H synthase 2 (EC 1.14.99.1) (Cyclooxygenase-2) (COX-2) (PHS II) (Prostaglandin H2 synthase 2) (PGH synthase 2) (PGHS-2) (Prostaglandin-endoperoxide synthase 2), PTGS2, COX2 |
 | Immunofluorescence staining of HepG2 Cells with RACO0524 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in U-87 whole cell lysate All lanes COX2 Antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 69 kDa. Observed band size: 69 kDa. |
| UniProt Protein Function: | COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.14.99.1 Chromosomal Location of Human Ortholog: 1q25.2-q25.3 Cellular Component: endoplasmic reticulum membrane; neuron projection; protein complex; cytoplasm; caveola; nucleus Molecular Function:prostaglandin-endoperoxide synthase activity; enzyme binding; peroxidase activity; protein homodimerization activity; arachidonate 15-lipoxygenase activity; metal ion binding; heme binding; lipid binding Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; response to lithium ion; positive regulation of smooth muscle cell proliferation; response to glucocorticoid stimulus; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; decidualization; sensory perception of pain; prostaglandin biosynthetic process; negative regulation of synaptic transmission, dopaminergic; positive regulation of synaptic transmission, glutamatergic; response to estradiol stimulus; bone mineralization; negative regulation of cell cycle; negative regulation of cell proliferation; response to vitamin D; lipoxygenase pathway; nicotinamide metabolic process; negative regulation of smooth muscle contraction; regulation of blood pressure; arachidonic acid metabolic process; positive regulation of smooth muscle contraction; angiogenesis; inflammatory response; response to fructose stimulus; water-soluble vitamin metabolic process; positive regulation of prostaglandin biosynthetic process; response to drug; negative regulation of calcium ion transport; vitamin metabolic process; cyclooxygenase pathway; positive regulation of synaptic plasticity; learning; memory; prostaglandin metabolic process; hair cycle; response to manganese ion; ovulation; NAD metabolic process; regulation of inflammatory response; positive regulation of vasoconstriction; positive regulation of fever; brown fat cell differentiation; response to oxidative stress; cell motility; embryo implantation |
| NCBI Summary: | Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009] |
| UniProt Code: | P35354 |
| NCBI GenInfo Identifier: | 3915797 |
| NCBI Gene ID: | 5743 |
| NCBI Accession: | P35354.2 |
| UniProt Secondary Accession: | P35354,Q16876, A8K802, |
| UniProt Related Accession: | P35354 |
| Molecular Weight: | |
| NCBI Full Name: | Prostaglandin G/H synthase 2 |
| NCBI Synonym Full Names: | prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) |
| NCBI Official Symbol: | PTGS2 |
| NCBI Official Synonym Symbols: | COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS |
| NCBI Protein Information: | prostaglandin G/H synthase 2; PHS II; PGH synthase 2; cyclooxygenase 2b; prostaglandin H2 synthase 2 |
| UniProt Protein Name: | Prostaglandin G/H synthase 2 |
| UniProt Synonym Protein Names: | Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2 |
| Protein Family: | Prostaglandin G/H synthase |
| UniProt Gene Name: | PTGS2 |
| UniProt Entry Name: | PGH2_HUMAN |