Anti-PSPC1 Antibody (CAB9209)
- SKU:
- CAB9209
- Product type:
- Antibody
- Application:
- WB
- Application:
- IP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PSPC1 Antibody |
| 抗体コード: | CAB9209 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human PSPC1 (NP_001035879.1). |
| 申し込み: | WB IHC IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | BxPC-3, A-549, U-87MG, Mouse brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human PSPC1 (NP_001035879.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MMLR GNLK QVRI EKNP ARLR ALES AVGE SEPA AAAA MALA LAGE PAPP APAP PEDH PDEE MGFT IDIK SF |
| 遺伝子ID: | 55269 |
| Uniprot: | Q8WXF1 |
| セルラーロケーション: | Cytoplasm, Nucleus, Nucleus matrix, Nucleus speckle, nucleolus |
| 計算された分子量: | 45kDa/58kDa |
| 観察された分子量: | 65kDa |
| 同義語: | PSPC1, PSP1 |
| バックグラウンド: | This gene encodes a nucleolar protein that localizes to punctate subnuclear structures that occur close to splicing speckles, known as paraspeckles. These paraspeckles are composed of RNA-protein structures that include a non-coding RNA, NEAT1/Men epsilon/beta, and the Drosophila Behavior Human Splicing family of proteins, which include the product of this gene and the P54NRB/NONO and PSF/SFPQ proteins. Paraspeckles may function in the control of gene expression via an RNA nuclear retention mechanism. The protein encoded by this gene is found in paraspeckles in transcriptionally active cells, but it localizes to unique cap structures at the nucleolar periphery when RNA polymerase II transcription is inhibited, or during telophase. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene, which is also located on chromosome 13, has been identified. |
| UniProt Protein Function: | PSPC1: Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line. Binds to poly(A), poly(G) and poly(U) RNA homopolymers. Together with NONO, required for the formation of nuclear paraspeckles. Belongs to the PSPC family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA-binding; Nucleolus Chromosomal Location of Human Ortholog: 13q12.11 Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nucleolus; nuclear speck; paraspeckles Molecular Function:protein binding; nucleotide binding Biological Process: transcription, DNA-dependent; rhythmic process; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm |
| NCBI Summary: | This gene encodes a nucleolar protein that localizes to punctate subnuclear structures that occur close to splicing speckles, known as paraspeckles. These paraspeckles are composed of RNA-protein structures that include a non-coding RNA, NEAT1/Men epsilon/beta, and the Drosophila Behavior Human Splicing family of proteins, which include the product of this gene and the P54NRB/NONO and PSF/SFPQ proteins. Paraspeckles may function in the control of gene expression via an RNA nuclear retention mechanism. The protein encoded by this gene is found in paraspeckles in transcriptionally active cells, but it localizes to unique cap structures at the nucleolar periphery when RNA polymerase II transcription is inhibited, or during telophase. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene, which is also located on chromosome 13, has been identified. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q8WXF1 |
| NCBI GenInfo Identifier: | 109240550 |
| NCBI Gene ID: | 55269 |
| NCBI Accession: | NP_001035879.1 |
| UniProt Secondary Accession: | Q8WXF1,Q5JTQ3, Q8NCZ9, Q8WXE8, Q9NV36, |
| UniProt Related Accession: | Q8WXF1 |
| Molecular Weight: | 58,744 Da |
| NCBI Full Name: | paraspeckle component 1 |
| NCBI Synonym Full Names: | paraspeckle component 1 |
| NCBI Official Symbol: | PSPC1 |
| NCBI Official Synonym Symbols: | PSP1 |
| NCBI Protein Information: | paraspeckle component 1; paraspeckle protein 1 |
| UniProt Protein Name: | Paraspeckle component 1 |
| UniProt Synonym Protein Names: | Paraspeckle protein 1 |
| Protein Family: | Paraspeckle component |
| UniProt Gene Name: | PSPC1 |
| UniProt Entry Name: | PSPC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PSPC1 antibody (CAB9209) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded Mouse testis using PSPC1 Rabbit pAb (CAB9209) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat brain using PSPC1 Rabbit pAb (CAB9209) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human esophageal using PSPC1 Rabbit pAb (CAB9209) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of A-549 cells using 3ug PSPC1 antibody (CAB9209). Western blot was performed from the immunoprecipitate using PSPC1 antibody (CAB9209) at a dilition of 1:1000. |
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