Anti-PSMA5 Antibody (CAB14053)
- SKU:
- CAB14053
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PSMA5 Antibody |
| 抗体コード: | CAB14053 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human PSMA5 (NP_002781.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, 293T, HepG2, U-251MG, BxPC-3, Mouse liver, Mouse thymus, Mouse heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human PSMA5 (NP_002781.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MFLT RSEY DRGV NTFS PEGR LFQV EYAI EAIK LGST AIGI QTSE GVCL AVEK RITS PLME PSSI EKIV EIDA HIGC AMSG LIAD AKTL IDKA RVET QNHW FTYN ETMT VESV TQAV SNLA LQFG EEDA DPGA MSRP FGVA LLFG GVDE KGPQ LFHM DPSG TFVQ CDAR AIGS ASEG AQSS LQEV YHKS MTLK EAIK SSLI ILKQ VMEE KLNA TNIE LATV QPGQ NFHM FTKE ELEE VIKD I |
| 遺伝子ID: | 5686 |
| Uniprot: | P28066 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 20kDa/26kDa |
| 観察された分子量: | 26kDa |
| 同義語: | PSMA5, PSC5, ZETA |
| バックグラウンド: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. |
| UniProt Protein Function: | PSMA5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Up-regulated in colon cancer cell lines. Up-regulated in fetal Down syndrome (DS) brain. May be the target of the transcriptional activator NFE2L2. Expressed in fetal brain. Belongs to the peptidase T1A family. |
| UniProt Protein Details: | Protein type:EC 3.4.25.1; Proteasome complex; Protease Chromosomal Location of Human Ortholog: 1p13 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; proteasome complex; proteasome core complex Molecular Function:protein binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway |
| NCBI Summary: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010] |
| UniProt Code: | P28066 |
| NCBI GenInfo Identifier: | 38258905 |
| NCBI Gene ID: | 5686 |
| NCBI Accession: | P28066.3 |
| UniProt Secondary Accession: | P28066,Q3T1C1, Q6IBF7, B2R8F6, B4E2V4, |
| UniProt Related Accession: | P28066 |
| Molecular Weight: | 20,035 Da |
| NCBI Full Name: | Proteasome subunit alpha type-5 |
| NCBI Synonym Full Names: | proteasome subunit alpha 5 |
| NCBI Official Symbol: | PSMA5 |
| NCBI Official Synonym Symbols: | PSC5; ZETA |
| NCBI Protein Information: | proteasome subunit alpha type-5 |
| UniProt Protein Name: | Proteasome subunit alpha type-5 |
| UniProt Synonym Protein Names: | Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome zeta chain |
| UniProt Gene Name: | PSMA5 |
| UniProt Entry Name: | PSA5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PSMA5 antibody (CAB14053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat lung using PSMA5 Antibody (CAB14053) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using PSMA5 Antibody (CAB14053) at dilution of 1:100 (40x lens). |
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