Anti-PRMT5 抗体 (CAB1520)

抗体名:	Anti-PRMT5 Antibody
抗体コード:	CAB1520
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	A synthetic peptide corresponding to a sequence within amino acids 40-140 of human PRMT5 (NP_006100.2).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	Jurkat, HepG2, HeLa, NIH/3T3, PC-12, COS-1

免疫原:	A synthetic peptide corresponding to a sequence within amino acids 40-140 of human PRMT5 (NP_006100.2).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	FLCM PVFH PRFK REFI QEPA KNRP GPQT RSDL LLSG RDWN TLIV GKLS PWIR PDSK VEKI RRNS EAAM LQEL NFGA YLGL PAFL LPLN QEDN TNLA RVLT N
遺伝子ID:	10419
Uniprot:	O14744
セルラーロケーション:	Cytoplasm, Golgi apparatus, Nucleus
計算された分子量:	53kDa/66kDa/67kDa/71kDa/72kDa
観察された分子量:	70kDa

同義語:

HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs, PRMT5

バックグラウンド:

This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	PRMT5: an ubiquitous enzyme with protein methyltransferase activity. Methylates specific arginine residues in the transcription elongation factor SPT5, and the small nuclear ribonucleoproteins SNRPD1 and SNRPD3. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SPT5 transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Component of the methylosome, a 20S complex that includes ICLN, SKB1 and WDR77. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SPT5. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.
UniProt Protein Details:	Protein type:EC 2.1.1.125; Cell cycle regulation; Methyltransferase, protein arginine; RNA processing; Methyltransferase Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus Molecular Function:methyltransferase activity; ribonucleoprotein binding; protein binding; protein-arginine omega-N symmetric methyltransferase activity; histone-arginine N-methyltransferase activity; chromatin binding; transcription corepressor activity Biological Process: cell proliferation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; establishment and/or maintenance of chromatin architecture; peptidyl-arginine methylation; spliceosomal snRNP biogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of mitosis; gene expression; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; peptidyl-arginine N-methylation; endothelial cell activation
NCBI Summary:	This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
UniProt Code:	O14744
NCBI GenInfo Identifier:	32171585
NCBI Gene ID:	10419
NCBI Accession:	O14744.4
UniProt Related Accession:	O14744
Molecular Weight:
NCBI Full Name:	Protein arginine N-methyltransferase 5
NCBI Synonym Full Names:	protein arginine methyltransferase 5
NCBI Official Symbol:	PRMT5
NCBI Official Synonym Symbols:	HSL7; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
NCBI Protein Information:	protein arginine N-methyltransferase 5
UniProt Protein Name:	Protein arginine N-methyltransferase 5
UniProt Synonym Protein Names:	72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5 (EC:2.1.1.125); Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog; SKB1 homolog; SKB1Hs
UniProt Gene Name:	PRMT5
UniProt Entry Name:	ANM5_HUMAN

	Western blot analysis of extracts of various cell lines, using PRMT5 antibody (CAB1520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Immunohistochemistry of paraffin-embedded rat brain using PRMT5 antibody (CAB1520) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PRMT5 antibody (CAB1520) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using PRMT5 antibody (CAB1520) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of H9C2 cells using PRMT5 Rabbit pAb (CAB1520) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-PRMT5 Antibody (CAB19533)

Anti-PRMT5 Antibody (CAB2290)

PRMT5 Antibody (PACO11486)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)