Anti-PRMT3 Antibody (CAB13068)[KO Validated]

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SKU:
CAB13068
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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Description

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抗体名:Anti-PRMT3 Antibody [KO Validated]
抗体コード:CAB13068
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB
反応性:Human, Mouse
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PRMT3 (NP_005779.1).
申し込み:WB
推奨希釈:WB 1:1000 - 1:2000
反応性:Human, Mouse
ポジティブサンプル:Mouse brain, 293T
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PRMT3 (NP_005779.1).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:MCSL ASGA TGGR GAVE NEED LPEL SDSG DEAA WEDE DDAD LPHG KQQT PCLF CNRL FTSA EETF SHCK SEHQ FNID SMVH KHGL EFYG YIKL INFI RLKN PTVE YMNS IYNP VPWE KEEY LKPV LEDD LLLQ FDVE DLYE PVSV PFSY PNGL SENT SVVE KLKH MEAR ALSA EAAL ARAR EDLQ KMKQ FAQD FVMH TDVR TCSS STSV IADL QEDE DGVY
遺伝子ID:10196
Uniprot:O60678
セルラーロケーション:Cytoplasm
計算された分子量:52kDa/59kDa
観察された分子量:60kDa
同義語:PRMT3, HRMT1L3
バックグラウンド:This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PRMT3: Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins. Belongs to the protein arginine N-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:C2H2-type zinc finger protein; EC 2.1.1.-; Methyltransferase; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytosol

Molecular Function:histone-arginine N-methyltransferase activity; methyltransferase activity; protein binding; protein-arginine omega-N asymmetric methyltransferase activity

Biological Process: negative regulation of protein ubiquitination; regulation of transcription, DNA-dependent

NCBI Summary:This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
UniProt Code:O60678
NCBI GenInfo Identifier:44771198
NCBI Gene ID:10196
NCBI Accession:NP_005779.1
UniProt Related Accession:O60678
Molecular Weight:52kDa; 59kDa
NCBI Full Name:protein arginine N-methyltransferase 3 isoform 1
NCBI Synonym Full Names:protein arginine methyltransferase 3
NCBI Official Symbol:PRMT3  
NCBI Official Synonym Symbols:HRMT1L3  
NCBI Protein Information:protein arginine N-methyltransferase 3
UniProt Protein Name:Protein arginine N-methyltransferase 3
UniProt Synonym Protein Names:Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
Protein Family:Protein arginine N-methyltransferase
UniProt Gene Name:PRMT3  
UniProt Entry Name:ANM3_HUMAN
Anti-PRMT3 Antibody [KO Validated] (CAB13068)Western blot analysis of extracts of mouse brain, using PRMT3 antibody (CAB13068) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PRMT3 Antibody [KO Validated] (CAB13068)Western blot analysis of extracts from normal (control) and PRMT3 Rabbit pAb knockout (KO) 293T cells, using PRMT3 Rabbit pAb antibody (CAB13068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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