Anti-PRKG1 Antibody (CAB2565)
- SKU:
- CAB2565
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PRKG1 Antibody |
| 抗体コード: | CAB2565 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PRKG1 (NP_006249.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HepG2 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PRKG1 (NP_006249.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGTL RDLQ YALQ EKIE ELRQ RDAL IDEL ELEL DQKD ELIQ KLQN ELDK YRSV IRPA TQQA QKQS ASTL QGEP RTKR QAIS AEPT AFDI QDLS HVTL PFYP KSPQ SKDL IKEA ILDN DFMK NLEL SQIQ EIVD CMYP VEYG KDSC IIKE GDVG SLVY VMED GKVE VTKE GVKL CTMG PGKV FGEL AILY NCTR TATV KTLV NVKL WAID RQCF QTIM MRTG LIKH TEYM EFLK SVPT FQSL PEEI LSKL ADVL EETH YENG EYII RQGA RGDT FFII SKGT VNVT REDS PSED PVFL RTLG |
| 遺伝子ID: | 5592 |
| Uniprot: | Q13976 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 44kDa/76kDa/77kDa |
| 観察された分子量: | 65kDa |
| 同義語: | PRKG1, AAT8, PKG, PRKG1B, PRKGR1B, cGK, cGK 1, cGK1, cGKI, cGKI-BETA, cGKI-alpha |
| バックグラウンド: | Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. |
| UniProt Protein Function: | PKG1: an AGC kinase of the PKG family. Type I cGMP-dependent protein kinase; relaxes vascular smooth muscle and inhibits platelet aggregation. Binding of cGMP results in enzyme activation. Two splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:AGC group; EC 2.7.11.12; Kinase, protein; PKG family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 10q11.23-q21.1 Cellular Component: cytoplasm; cytosol; plasma membrane Molecular Function:calcium channel regulator activity; cGMP-dependent protein kinase activity; protein binding; protein serine/threonine kinase activity Biological Process: actin cytoskeleton organization and biogenesis; regulation of GTPase activity; signal transduction |
| NCBI Summary: | Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q13976 |
| NCBI GenInfo Identifier: | 6225588 |
| NCBI Gene ID: | 5592 |
| NCBI Accession: | Q13976.3 |
| UniProt Secondary Accession: | Q13976,P14619, Q5JP05, Q5JSJ6, Q6P5T7, A5YM56, B3KSF3 E2PU10, |
| UniProt Related Accession: | Q13976 |
| Molecular Weight: | |
| NCBI Full Name: | cGMP-dependent protein kinase 1 |
| NCBI Synonym Full Names: | protein kinase, cGMP-dependent, type I |
| NCBI Official Symbol: | PRKG1 |
| NCBI Official Synonym Symbols: | PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha |
| NCBI Protein Information: | cGMP-dependent protein kinase 1 |
| UniProt Protein Name: | cGMP-dependent protein kinase 1 |
| UniProt Synonym Protein Names: | cGMP-dependent protein kinase I; cGKI |
| UniProt Gene Name: | PRKG1 |
 | Western blot analysis of extracts of HepG2 cells, using PRKG1 antibody (CAB2565) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
 | Immunohistochemistry of paraffin-embedded mouse lung using PRKG1 antibody (CAB2565) at dilution of 1:100 (40x lens). |
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