Anti-PRKAR1B Antibody (CAB10125)
- SKU:
- CAB10125
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PRKAR1B Antibody |
| 抗体コード: | CAB10125 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-290 of human PRKAR1B (NP_002726.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:4000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-251MG, HeLa, Jurkat, HepG2, BT-474, Mouse brain, Mouse lung, Rat brain, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 190-290 of human PRKAR1B (NP_002726.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | WVTN ISEG GSFG ELAL IYGT PRAA TVKA KTDL KLWG IDRD SYRR ILMG STLR KRKM YEEF LSKV SILE SLEK WERL TVAD ALEP VQFE DGEK IVVQ GEPG D |
| 遺伝子ID: | 5575 |
| Uniprot: | P31321 |
| セルラーロケーション: | Cell membrane |
| 計算された分子量: | 43kDa |
| 観察された分子量: | 43kDa |
| 同義語: | PRKAR1B, PRKAR1 |
| バックグラウンド: | The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | PKAR1B: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Belongs to the cAMP-dependent kinase regulatory chain family. |
| UniProt Protein Details: | Protein type:Protein kinase, regulatory subunit Chromosomal Location of Human Ortholog: 7p22 Cellular Component: cytosol Molecular Function:cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity Biological Process: activation of protein kinase A; blood coagulation; renal water homeostasis |
| NCBI Summary: | The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P31321 |
| NCBI GenInfo Identifier: | 229463042 |
| NCBI Gene ID: | 5575 |
| NCBI Accession: | P31321.4 |
| UniProt Secondary Accession: | P31321,Q8N422, |
| UniProt Related Accession: | P31321 |
| Molecular Weight: | 43,073 Da |
| NCBI Full Name: | cAMP-dependent protein kinase type I-beta regulatory subunit |
| NCBI Synonym Full Names: | protein kinase cAMP-dependent type I regulatory subunit beta |
| NCBI Official Symbol: | PRKAR1B |
| NCBI Official Synonym Symbols: | PRKAR1 |
| NCBI Protein Information: | cAMP-dependent protein kinase type I-beta regulatory subunit |
| UniProt Protein Name: | cAMP-dependent protein kinase type I-beta regulatory subunit |
| Protein Family: | cAMP-dependent protein kinase |
| UniProt Gene Name: | PRKAR1B |
| UniProt Entry Name: | KAP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PRKAR1B antibody (CAB10125) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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