Anti-PRDM1 Antibody (CAB1960)
- SKU:
- CAB1960
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-PRDM1 Antibody |
| 抗体コード: | CAB1960 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PRDM1 (NP_878911.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | LO2, BxPC-3, K-562, HepG2 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PRDM1 (NP_878911.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MEKI YSRG ELHH FIDG FNEE KSNW MRYV NPAH SPRE QNLA ACQN GMNI YFYT IKPI PANQ ELLV WYCR DFAE RLHY PYPG ELTM MNLT QTQS SLKQ PSTE KNEL CPKN VPKR EYSV KEIL KLDS NPSK GKDL YRSN ISPL TSEK DLDD FRRR GSPE MPFY PRVV YPIR APLP EDFL KASL AYGI ERPT YITR SPIP SSTT PSPS ARSS PDQS LKSS SPHS SPGN TVSP VGPG SQEH RDSY |
| 遺伝子ID: | 639 |
| Uniprot: | O75626 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 76kDa/87kDa/91kDa |
| 観察された分子量: | 100kDa |
| 同義語: | PRDM1, BLIMP1, PRDI-BF1 |
| バックグラウンド: | This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. |
| UniProt Protein Function: | PRDM1: a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene and can inhibit virus-mediated IFN-¿ production. Recruits chromatin-modifying enzymes including histone deacetylases and methyltransferases. Drives the maturation of B-lymphocytes into Ig secreting cells. Interacts with PRMT5. Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1. In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation and in a subset of T-cells suggesting that it may play a wider role in homeostasis and differentiation. Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc, CIITA, Pax5, Spi-B, and Id3.Three isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription regulation; Transcription, coactivator/corepressor; C2H2-type zinc finger protein; Methyltransferase, protein lysine, predicted; DNA-binding Chromosomal Location of Human Ortholog: 6q21 Cellular Component: cytoplasm; nucleoplasm Molecular Function:protein binding; transcription factor activity Biological Process: negative regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75626 |
| NCBI GenInfo Identifier: | 296452869 |
| NCBI Gene ID: | 639 |
| NCBI Accession: | O75626.2 |
| UniProt Secondary Accession: | O75626,Q86WM7, B2REA6, E1P5E0, |
| UniProt Related Accession: | O75626 |
| Molecular Weight: | 76,835 Da |
| NCBI Full Name: | PR domain zinc finger protein 1 |
| NCBI Synonym Full Names: | PR/SET domain 1 |
| NCBI Official Symbol: | PRDM1 |
| NCBI Official Synonym Symbols: | BLIMP1; PRDI-BF1 |
| NCBI Protein Information: | PR domain zinc finger protein 1 |
| UniProt Protein Name: | PR domain zinc finger protein 1 |
| UniProt Synonym Protein Names: | BLIMP-1; Beta-interferon gene positive regulatory domain I-binding factor; PR domain-containing protein 1; Positive regulatory domain I-binding factor 1; PRDI-BF1; PRDI-binding factor 1 |
| Protein Family: | PR domain zinc finger protein |
| UniProt Gene Name: | PRDM1 |
| UniProt Entry Name: | PRDM1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PRDM1 antibody (CAB1960) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |