Anti-PPP4R1 Antibody (CAB8361)
- SKU:
- CAB8361
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PPP4R1 Antibody |
| 抗体コード: | CAB8361 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IP |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1). |
| 申し込み: | WB IHC IP |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T, U-251MG, BT-474, K-562, SKOV3 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | NPSS SGQY FKEE SKSS EEMS VENK NRTR DQEA PEDV QVRP EDTP SDLS VSNS SVIL ENTM EDHA AEAS GKPL GEIS VPLD SSLL CTLS SESH QEAA SNEN DKKP GNYK SMLR PEVG TTSQ DSAL LDQE LYNS FHFW RTPL PEID LDIE LEQN SGGK PSPE GPEE ESEG PVPS SPNI TMAT RKEL EEMI ENLE PHID DPDV KAQV EVLS AALR ASSL DAHE ETIS IEKR SDLQ DELD INEL PNCK INQE DSVP LISD AVEN MDST LHYI HSDS DLSN NSSF SPDE ERRT KVQD VVPQ ALLD QYLS MTDP SRAQ TVDT EIAK HCAY |
| 遺伝子ID: | 9989 |
| Uniprot: | Q8TF05 |
| セルラーロケーション: | |
| 計算された分子量: | 105kDa/107kDa |
| 観察された分子量: | 130kDa |
| 同義語: | PPP4R1, MEG1, PP4(Rmeg), PP4R1 |
| バックグラウンド: | This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. |
| NCBI Summary: | This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012] |
| UniProt Code: | Q8TF05 |
| NCBI GenInfo Identifier: | 108936953 |
| NCBI Gene ID: | 9989 |
| NCBI Accession: | NP_001035847 |
| UniProt Related Accession: | Q8TF05 |
| Molecular Weight: | |
| NCBI Full Name: | serine/threonine-protein phosphatase 4 regulatory subunit 1 isoform a |
| NCBI Synonym Full Names: | protein phosphatase 4 regulatory subunit 1 |
| NCBI Official Symbol: | PPP4R1 |
| NCBI Official Synonym Symbols: | MEG1; PP4R1; PP4(Rmeg) |
| NCBI Protein Information: | serine/threonine-protein phosphatase 4 regulatory subunit 1 |
| UniProt Protein Name: | Serine/threonine-protein phosphatase 4 regulatory subunit 1 |
| UniProt Gene Name: | PPP4R1 |
| UniProt Entry Name: | PP4R1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PPP4R1 antibody (CAB8361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded human liver using PPP4R1 antibody (CAB8361) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using PPP4R1 antibody (CAB8361) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug PPP4R1 antibody (CAB8361). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (CAB8361) at a dilition of 1:1000. |
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