Anti-PPP1R14A Antibody (CAB10160)
- SKU:
- CAB10160
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PPP1R14A Antibody |
| 抗体コード: | CAB10160 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human PPP1R14A (NP_150281.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | K-562, Mouse lung, Mouse brain, Mouse spleen, Rat lung, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human PPP1R14A (NP_150281.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAAQ RLGK RVLS KLQS PSRA RGPG GSPG GLQK RHAR VTVK YDRR ELQR RLDV EKWI DGRL EELY RGME ADMP DEIN IDEL LELE SEEE RSRK IQGL LKSC GKPV EDFI QELL AKLQ GLHR QPGL RQPS PSHD GSLS PLQD RART AHP |
| 遺伝子ID: | 94274 |
| Uniprot: | Q96A00 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 13kDa/16kDa |
| 観察された分子量: | 19KDa |
| 同義語: | PPP1R14A, CPI-17, CPI17, PPP1INL |
| バックグラウンド: | The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
| UniProt Protein Function: | PPP1R14A: a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity. Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2 ) concentration. Involved in the regulation of cell morphology, re-organisation of microfilaments, and cell spreading. Also expressed in human platelets and in brain where it may play a role in cerebellar long-term synaptic depression. Phosphorylation by ILK or Rho kinase enhances its ability to inhibit PP-1A. |
| UniProt Protein Details: | Protein type:Inhibitor; Protein phosphatase, regulatory subunit Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: cytosol Molecular Function:protein phosphatase inhibitor activity Biological Process: negative regulation of catalytic activity; regulation of phosphorylation; small GTPase mediated signal transduction |
| NCBI Summary: | The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q96A00 |
| NCBI GenInfo Identifier: | 55583974 |
| NCBI Gene ID: | 94274 |
| NCBI Accession: | Q96A00.1 |
| UniProt Secondary Accession: | Q96A00,Q7Z4X7, Q96S54, |
| UniProt Related Accession: | Q96A00 |
| Molecular Weight: | 13,480 Da |
| NCBI Full Name: | Protein phosphatase 1 regulatory subunit 14A |
| NCBI Synonym Full Names: | protein phosphatase 1 regulatory inhibitor subunit 14A |
| NCBI Official Symbol: | PPP1R14A |
| NCBI Official Synonym Symbols: | CPI17; CPI-17; PPP1INL |
| NCBI Protein Information: | protein phosphatase 1 regulatory subunit 14A |
| UniProt Protein Name: | Protein phosphatase 1 regulatory subunit 14A |
| UniProt Synonym Protein Names: | 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17 |
| UniProt Gene Name: | PPP1R14A |
| UniProt Entry Name: | PP14A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PPP1R14A Rabbit pAb (CAB10160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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