Anti-PPCS Antibody (CAB12843)
- SKU:
- CAB12843
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PPCS Antibody |
| 抗体コード: | CAB12843 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human PPCS (NP_078940.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, OVCAR3, HeLa, HepG2, 293T, Mouse brain, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human PPCS (NP_078940.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAEM DPVA EFPQ PPGA ARWA EVMA RFAA RLGA QGRR VVLV TSGG TKVP LEAR PVRF LDNF SSGR RGAT SAEA FLAA GYGV LFLY RARS AFPY AHRF PPQT WLSA LRPS GPAL SGLL SLEA EENA LPGF AEAL RSYQ EAAA AGTF LAVE FTTL ADYL HLLQ AAAQ ALNP LGPS AMFY LAAA VSDF YVPV SEMP EHKI QSSG GPLQ ITMK MVPK LLSP LVKD WAPK AFII SFKL ETDP AIVI NRAR KALE IYQH QVVV ANIL ESRQ SFVF IVTK DSET KLLL SEEE IEKG VEIE EKIV DNLQ SRHT AFIG DRN |
| 遺伝子ID: | 79717 |
| Uniprot: | Q9HAB8 |
| セルラーロケーション: | |
| 計算された分子量: | 15kDa/34kDa |
| 観察された分子量: | 34kDa |
| 同義語: | PPCS |
| バックグラウンド: |
| UniProt Protein Function: | PPCS: Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'- phosphopantothenate to form 4-phosphopantothenoylcysteine. Belongs to the PPC synthetase family. |
| UniProt Protein Details: | Protein type:Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 6.3.2.5; Ligase Chromosomal Location of Human Ortholog: 1p34.2 Cellular Component: cytosol Molecular Function:phosphopantothenate--cysteine ligase activity Biological Process: coenzyme A biosynthetic process; coenzyme biosynthetic process |
| NCBI Summary: | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9HAB8 |
| NCBI GenInfo Identifier: | 47117318 |
| NCBI Gene ID: | 79717 |
| NCBI Accession: | Q9HAB8.2 |
| UniProt Secondary Accession: | Q9HAB8,Q3KQT2, Q5VVM0, |
| UniProt Related Accession: | Q9HAB8 |
| Molecular Weight: | 34kDa |
| NCBI Full Name: | Phosphopantothenate--cysteine ligase |
| NCBI Synonym Full Names: | phosphopantothenoylcysteine synthetase |
| NCBI Official Symbol: | PPCS |
| NCBI Protein Information: | phosphopantothenate--cysteine ligase |
| UniProt Protein Name: | Phosphopantothenate--cysteine ligase |
| UniProt Synonym Protein Names: | Phosphopantothenoylcysteine synthetase; PPC synthetase |
| Protein Family: | Phosphopantothenate--cysteine ligase |
| UniProt Gene Name: | PPCS |
 | Western blot analysis of extracts of various cell lines, using PPCS antibody (CAB12843) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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