Anti-POLR3K Antibody (CAB6467)
- SKU:
- CAB6467
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-POLR3K Antibody |
| 抗体コード: | CAB6467 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human POLR3K (NP_057394.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | MCF7, SW620 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human POLR3K (NP_057394.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MLLF CPGC GNGL IVEE GQRC HRFA CNTC PYVH NITR KVTN RKYP KLKE VDDV LGGA AAWE NVDS TAES CPKC EHPR AYFM QLQT RSAD EPMT TFYK CCNA QCGH RWRD |
| 遺伝子ID: | 51728 |
| Uniprot: | Q9Y2Y1 |
| セルラーロケーション: | Nucleus, nucleolus |
| 計算された分子量: | 12kDa |
| 観察された分子量: | 12kDa |
| 同義語: | POLR3K, C11, C11-RNP3, My010, RPC10, RPC11, RPC12.5 |
| バックグラウンド: | This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17. |
| UniProt Protein Function: | POLR3K: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. |
| UniProt Protein Details: | Protein type:Transcription initiation complex; DNA-binding; Transferase Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol Molecular Function:DNA binding; zinc ion binding; DNA-directed RNA polymerase activity Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; positive regulation of interferon type I production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter |
| NCBI Summary: | This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.[provided by RefSeq, Jul 2010] |
| UniProt Code: | Q9Y2Y1 |
| NCBI GenInfo Identifier: | 116242768 |
| NCBI Gene ID: | 51728 |
| NCBI Accession: | Q9Y2Y1.2 |
| UniProt Secondary Accession: | Q9Y2Y1,Q1W6H4, Q96S35, |
| UniProt Related Accession: | Q9Y2Y1 |
| Molecular Weight: | 108 |
| NCBI Full Name: | DNA-directed RNA polymerase III subunit RPC10 |
| NCBI Synonym Full Names: | polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa |
| NCBI Official Symbol: | POLR3K |
| NCBI Official Synonym Symbols: | C11; My010; RPC10; RPC11; RPC12.5; C11-RNP3 |
| NCBI Protein Information: | DNA-directed RNA polymerase III subunit RPC10; RNA polymerase III subunit C10; RNA polymerase III subunit C11; RNA polymerase III subunit CII; RNA polymerase III 12.5 kDa subunit; DNA-directed RNA polymerase III subunit K; DNA-directed RNA polymerases III 12.5 kDa polypeptide |
| UniProt Protein Name: | DNA-directed RNA polymerase III subunit RPC10 |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase III subunit K; RNA polymerase III 12.5 kDa subunit; RPC12.5; RNA polymerase III subunit C11; HsC11p; RPC11; hRPC11 |
| Protein Family: | DNA-directed RNA polymerase |
| UniProt Gene Name: | POLR3K |
| UniProt Entry Name: | RPC10_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POLR3K antibody (CAB6467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |