Anti-POLR3F Antibody (CAB12224)
- SKU:
- CAB12224
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-POLR3F Antibody |
| 抗体コード: | CAB12224 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human POLR3F (NP_006457.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, HeLa, K-562, Mouse lung, Mouse spleen, Mouse testis, Rat spleen |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human POLR3F (NP_006457.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAEV KVKV QPPD ADPV EIEN RIIE LCHQ FPHG ITDQ VIQN EMPH IEAQ QRAV AINR LLSM GQLD LLRS NTGL LYRI KDSQ NAGK MKGS DNQE KLVY QIIE DAGN KGIW SRDI RYKS NLPL TEIN KILK NLES KKLI KAVK SVAA SKKK VYML YNLQ PDRS VTGG AWYS DQDF ESEF VEVL NQQC FKFL QSKA ETAR ESKQ NPMI QRNS SFAS SHEV WKYI |
| 遺伝子ID: | 10621 |
| Uniprot: | Q9H1D9 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 35kDa |
| 観察された分子量: | 36kDa |
| 同義語: | POLR3F, RPC39, RPC6 |
| バックグラウンド: | The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | POLR3F: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transcription initiation complex Chromosomal Location of Human Ortholog: 20p11.23 Cellular Component: cytosol; DNA-directed RNA polymerase III complex; nucleoplasm Molecular Function:protein binding Biological Process: positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production |
| NCBI Summary: | The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
| UniProt Code: | Q9H1D9 |
| NCBI GenInfo Identifier: | 20139728 |
| NCBI Gene ID: | 10621 |
| NCBI Accession: | Q9H1D9.1 |
| UniProt Secondary Accession: | Q9H1D9,O15319, A8K4C7, |
| UniProt Related Accession: | Q9H1D9 |
| Molecular Weight: | 36kDa |
| NCBI Full Name: | DNA-directed RNA polymerase III subunit RPC6 |
| NCBI Synonym Full Names: | RNA polymerase III subunit F |
| NCBI Official Symbol: | POLR3F |
| NCBI Official Synonym Symbols: | RPC6; RPC39 |
| NCBI Protein Information: | DNA-directed RNA polymerase III subunit RPC6 |
| UniProt Protein Name: | DNA-directed RNA polymerase III subunit RPC6 |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase III subunit F; RNA polymerase III 39 kDa subunit; RPC39 |
| UniProt Gene Name: | POLR3F |
 | Western blot analysis of extracts of various cell lines, using POLR3F antibody (CAB12224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
View AllClose