Anti-POLR2J Antibody (CAB13627)
- SKU:
- CAB13627
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-POLR2J Antibody |
| 抗体コード: | CAB13627 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Mouse skeletal muscle |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MNAP PAFE SFLL FEGE KKIT INKD TKVP NACL FTIN KEDH TLGN IIKS QLLK DPQV LFAG YKVP HPLE HKII IRVQ TTPD YSPQ EAFT NAIT DLIS ELSL LEER FRVA IKDK QEGI E |
| 遺伝子ID: | 5439 |
| Uniprot: | P52435 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 13kDa |
| 観察された分子量: | 13kDa |
| 同義語: | POLR2J, POLR2J1, RPB11, RPB11A, RPB11m, hRPB14 |
| バックグラウンド: | This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. |
| UniProt Protein Function: | POLR2J: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft. Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family. |
| UniProt Protein Details: | Protein type:Transferase; DNA-binding; RNA splicing; RNA processing Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: DNA-directed RNA polymerase II, core complex; nucleoplasm; nucleus Molecular Function:DNA-directed RNA polymerase activity; LRR domain binding; protein binding Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA capping; nuclear mRNA splicing, via spliceosome; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; RNA-mediated gene silencing; snRNA transcription from RNA polymerase II promoter; somatic stem cell maintenance; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair |
| NCBI Summary: | This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P52435 |
| NCBI GenInfo Identifier: | 1710661 |
| NCBI Gene ID: | 5439 |
| NCBI Accession: | P52435.1 |
| UniProt Secondary Accession: | P52435,O43375, A5D6V8, |
| UniProt Related Accession: | P52435 |
| Molecular Weight: | 13,293 Da |
| NCBI Full Name: | DNA-directed RNA polymerase II subunit RPB11-a |
| NCBI Synonym Full Names: | RNA polymerase II subunit J |
| NCBI Official Symbol: | POLR2J |
| NCBI Official Synonym Symbols: | RPB11; RPB11A; RPB11m; hRPB14; POLR2J1 |
| NCBI Protein Information: | DNA-directed RNA polymerase II subunit RPB11-a |
| UniProt Protein Name: | DNA-directed RNA polymerase II subunit RPB11-a |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit |
| Protein Family: | DNA-directed RNA polymerase |
| UniProt Gene Name: | POLR2J |
| UniProt Entry Name: | RPB11_HUMAN |
 | Western blot analysis of extracts of mouse skeletal muscle, using POLR2J antibody (CAB13627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of A549 cells using POLR2J antibody (CAB13627). |
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