Anti-POLE3 Antibody (CAB6469)[KO Validated]
![Anti-POLE3 Antibody (CAB6469)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/25570/25418/anti-pole3-antibody-cab6469ko-validated__57245__73892.1645806294.jpg?c=1 "Anti-POLE3 Antibody (CAB6469)[KO Validated]")
- SKU:
- CAB6469
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
![Anti-POLE3 Antibody (CAB6469)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/25570/25418/anti-pole3-antibody-cab6469ko-validated__57245__73892.1645806294.jpg?c=1&imbypass=on "Anti-POLE3 Antibody (CAB6469)[KO Validated]")
Frequently bought together:
Description
| 抗体名: | Anti-POLE3 Antibody [KO Validated] |
| 抗体コード: | CAB6469 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HepG2, Mouse testis, Mouse bone marrow, Mouse spleen, 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAER PEDL NLPN AVIT RIIK EALP DGVN ISKE ARSA ISRA ASVF VLYA TSCA NNFA MKGK RKTL NASD VLSA MEEM EFQR FVTP LKEA LEAY RREQ KGKK EASE QKKK DKDK KTDS EEQD KSRD EDND EDEE RLEE EEQN EEEE VDN |
| 遺伝子ID: | 54107 |
| Uniprot: | Q9NRF9 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 16kDa |
| 観察された分子量: | 20-25kDa |
| 同義語: | POLE3, CHARAC17, CHRAC17, YBL1, p17 |
| バックグラウンド: | POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004] |
| UniProt Protein Function: | POLE3: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. |
| UniProt Protein Details: | Protein type:Nucleotide Metabolism - pyrimidine; EC 2.7.7.7; DNA replication; Nucleotide Metabolism - purine; Transferase Chromosomal Location of Human Ortholog: 9q33 Cellular Component: epsilon DNA polymerase complex; nucleus Molecular Function:protein binding Biological Process: DNA replication |
| NCBI Summary: | POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004] |
| UniProt Code: | Q9NRF9 |
| NCBI GenInfo Identifier: | 22653710 |
| NCBI Gene ID: | 54107 |
| NCBI Accession: | Q9NRF9.1 |
| UniProt Secondary Accession: | Q9NRF9,Q5W0U1, Q8N758, Q8NCE5, Q9NR32, |
| UniProt Related Accession: | Q9NRF9 |
| Molecular Weight: | 16,860 Da |
| NCBI Full Name: | DNA polymerase epsilon subunit 3 |
| NCBI Synonym Full Names: | DNA polymerase epsilon 3, accessory subunit |
| NCBI Official Symbol: | POLE3 |
| NCBI Official Synonym Symbols: | p17; YBL1; CHRAC17; CHARAC17 |
| NCBI Protein Information: | DNA polymerase epsilon subunit 3 |
| UniProt Protein Name: | DNA polymerase epsilon subunit 3 |
| UniProt Synonym Protein Names: | Arsenic-transactivated protein; AsTP; Chromatin accessibility complex 17 kDa protein; CHRAC-17; HuCHRAC17; DNA polymerase II subunit 3; DNA polymerase epsilon subunit p17 |
| Protein Family: | DNA polymerase |
| UniProt Gene Name: | POLE3 |
| UniProt Entry Name: | DPOE3_HUMAN |
![Anti-POLE3 Antibody [KO Validated] (CAB6469)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/t/874/A6469_1__68915.jpg) | Western blot analysis of extracts of various cell lines, using POLE3 antibody (CAB6469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
![Anti-POLE3 Antibody [KO Validated] (CAB6469)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/u/927/A6469_2__19355.jpg) | Western blot analysis of extracts from normal (control) and [KO Validated] POLE3 Rabbit pAb knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit pAb antibody (CAB6469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
![Anti-POLE3 Antibody [KO Validated] (CAB6469)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/r/316/A6469_3__95069.jpg) | Immunofluorescence analysis of U2OS cells using POLE3 antibody (CAB6469). |
![Anti-POLE3 Antibody [KO Validated] (CAB6469)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/z/466/A6469_4__52258.jpg) | Immunofluorescence analysis of U2OS cells using POLE3 antibody (CAB6469). (Green)gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser. |