Anti-PNP Antibody (CAB19886)[KO Validated]
![Anti-PNP Antibody (CAB19886)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/18556/18422/anti-pnp-antibody-cab19886ko-validated__27694__43628.1645803990.jpg?c=1 "Anti-PNP Antibody (CAB19886)[KO Validated]")
- SKU:
- CAB19886
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
![Anti-PNP Antibody (CAB19886)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/18556/18422/anti-pnp-antibody-cab19886ko-validated__27694__43628.1645803990.jpg?c=1&imbypass=on "Anti-PNP Antibody (CAB19886)[KO Validated]")
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Description
| 抗体名: | Anti-PNP Antibody [KO Validated] |
| 抗体コード: | CAB19886 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IP |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human PNP. |
| 申し込み: | WB IP |
| 推奨希釈: | WB 1:500 - 1:2000 IP 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T |
| 免疫原: | Recombinant protein of human PNP. |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 4860 |
| Uniprot: | P00491 |
| セルラーロケーション: | Cytoplasm, cytoskeleton |
| 計算された分子量: | 32kDa |
| 観察された分子量: | 30KDa |
![Anti-PNP Antibody [KO Validated] (CAB19886)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/v/735/A19886_1__32125.jpg) | Western blot analysis of extracts from normal (control) and PNP knockout (KO) 293T cells, using PNP antibody (CAB19886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| 同義語: | PNP, NP, PRO1837, PUNP |
| バックグラウンド: | This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. |
| UniProt Protein Function: | NP: a metabolic enzyme with purine-nucleoside phosphorylase activity. Defects are the cause of nucleoside phosphorylase deficiency (NP deficiency), with severe T-cell immunodeficiency with neurologic disorder in children. |
| UniProt Protein Details: | Protein type:Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Transferase; EC 2.4.2.1; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine Chromosomal Location of Human Ortholog: 14q13.1 Cellular Component: cytoplasm; cytosol; intracellular Molecular Function:drug binding; nucleoside binding; phosphate binding; purine binding; purine-nucleoside phosphorylase activity Biological Process: immune response; inosine catabolic process; nicotinamide riboside catabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; positive regulation of alpha-beta T cell differentiation; positive regulation of T cell proliferation; purine nucleotide catabolic process; purine salvage; response to drug Disease: Purine Nucleoside Phosphorylase Deficiency |
| NCBI Summary: | This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P00491 |
| NCBI GenInfo Identifier: | 108935929 |
| NCBI Gene ID: | 4860 |
| NCBI Accession: | P00491.2 |
| UniProt Secondary Accession: | P00491,Q15160, Q5PZ03, B2R8S5, D3DS00, |
| UniProt Related Accession: | P00491 |
| Molecular Weight: | 32,118 Da |
| NCBI Full Name: | Purine nucleoside phosphorylase |
| NCBI Synonym Full Names: | purine nucleoside phosphorylase |
| NCBI Official Symbol: | PNP |
| NCBI Official Synonym Symbols: | NP; PUNP; PRO1837 |
| NCBI Protein Information: | purine nucleoside phosphorylase |
| UniProt Protein Name: | Purine nucleoside phosphorylase |
| UniProt Synonym Protein Names: | Inosine phosphorylase; Inosine-guanosine phosphorylase |
| Protein Family: | Polyribonucleotide nucleotidyltransferase |
| UniProt Gene Name: | PNP |
| UniProt Entry Name: | PNPH_HUMAN |