Anti-PMEPA1 Antibody (CAB12171)
- SKU:
- CAB12171
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-PMEPA1 Antibody |
| 抗体コード: | CAB12171 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 173-252 of human PMEPA1 (NP_954638.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SKOV3, 22Rv1, 293T, Mouse brain, Rat brain, Rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 173-252 of human PMEPA1 (NP_954638.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | PSSN SGIS ATCY GSGG RMEG PPPT YSEV IGHY PGSS FQHQ QSSG PPSL LEGT RLHH THIA PLES AAIW SKEK DKQK GHPL |
| 遺伝子ID: | 56937 |
| Uniprot: | Q969W9 |
| セルラーロケーション: | Early endosome membrane, Golgi apparatus membrane, Single-pass membrane protein |
| 計算された分子量: | 26kDa/27kDa/31kDa |
| 観察された分子量: | 37kDa |
| 同義語: | PMEPA1, STAG1, TMEPAI |
| バックグラウンド: | This gene encodes a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins. Overexpression of this gene may play a role in multiple types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | TMEPAI: Involved in down-regulation of the androgen receptor (AR). Enhances ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4. Belongs to the PMEPA1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 20q13.31 Cellular Component: early endosome membrane; endosome membrane; Golgi membrane; intracellular membrane-bound organelle; plasma membrane Molecular Function:protein binding; WW domain binding Biological Process: negative regulation of transforming growth factor beta receptor signaling pathway |
| NCBI Summary: | This gene encodes a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins. Overexpression of this gene may play a role in multiple types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q969W9 |
| NCBI GenInfo Identifier: | 20140695 |
| NCBI Gene ID: | 56937 |
| NCBI Accession: | Q969W9.1 |
| UniProt Secondary Accession: | Q969W9,Q5TDR6, Q8NER4, Q96B72, Q9UJD3, |
| UniProt Related Accession: | Q969W9 |
| Molecular Weight: | 32kDa |
| NCBI Full Name: | Protein TMEPAI |
| NCBI Synonym Full Names: | prostate transmembrane protein, androgen induced 1 |
| NCBI Official Symbol: | PMEPA1 |
| NCBI Official Synonym Symbols: | STAG1; TMEPAI |
| NCBI Protein Information: | protein TMEPAI |
| UniProt Protein Name: | Protein TMEPAI |
| UniProt Synonym Protein Names: | Prostate transmembrane protein androgen induced 1; Solid tumor-associated 1 protein; Transmembrane prostate androgen-induced protein |
| Protein Family: | Protein |
| UniProt Gene Name: | PMEPA1 |
 | Western blot analysis of extracts of various cell lines, using PMEPA1 antibody (CAB12171) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using PMEPA1 antibody (CAB12171) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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