Anti-PLA2G6 Antibody (CAB9492)
- SKU:
- CAB9492
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PLA2G6 Antibody |
| 抗体コード: | CAB9492 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human PLA2G6 (NP_003551.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Rat |
| ポジティブサンプル: | Rat liver |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human PLA2G6 (NP_003551.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | RGSR PYES GPLE EFLK REFG EHTK MTDV RKPK VMLT GTLS DRQP AELH LFRN YDAP ETVR EPRF NQNV NLRP PAQP SDQL VWRA ARSS GAAP TYFR PNGR F |
| 遺伝子ID: | 8398 |
| Uniprot: | O60733 |
| セルラーロケーション: | Cytoplasm, Membrane, Peripheral membrane protein |
| 計算された分子量: | 46kDa/53kDa/84kDa/89kDa |
| 観察された分子量: | 105kDa |
| 同義語: | PLA2G6, CaI-PLA2, GVI, INAD1, IPLA2-VIA, NBIA2, NBIA2A, NBIA2B, PARK14, PLA2, PNPLA9, iPLA2, iPLA2beta |
| バックグラウンド: | The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only three of them have been determined to date. |
| UniProt Protein Function: | PLA2G6: Catalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F- actin polymerization at the pseudopods. Defects in PLA2G6 are the cause of neurodegeneration with brain iron accumulation type 2B (NBIA2B). A neurodegenerative disorder associated with iron accumulation in the brain, primarily in the basal ganglia. It is characterized by progressive extrapyramidal dysfunction leading to rigidity, dystonia, dysarthria and sensorimotor impairment. Defects in PLA2G6 are the cause of neurodegeneration with brain iron accumulation type 2A (NBIA2A); also known as Seitelberger disease. NBIA2A is a neurodegenerative disease characterized by pathologic axonal swelling and spheroid bodies in the central nervous system. Onset is within the first 2 years of life with death by age 10 years. Defects in PLA2G6 are the cause of Parkinson disease type 14 (PARK14). An adult-onset progressive neurodegenerative disorder characterized by parkinsonism, dystonia, severe cognitive decline, cerebral and cerebellar atrophy and absent iron in the basal ganglia on magnetic resonance imaging. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - linoleic acid; EC 3.1.1.4; Lipid Metabolism - arachidonic acid; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Phospholipase Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: cytoplasm; cytosol; extracellular space; microtubule organizing center Molecular Function:hydrolase activity; phospholipase A2 activity Biological Process: antibacterial humoral response; cardiolipin biosynthetic process Disease: Neurodegeneration With Brain Iron Accumulation 2a; Neurodegeneration With Brain Iron Accumulation 2b; Parkinson Disease 14, Autosomal Recessive |
| NCBI Summary: | The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells. Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only three of them have been determined to date. [provided by RefSeq, Dec 2010] |
| UniProt Code: | O60733 |
| NCBI GenInfo Identifier: | 6685712 |
| NCBI Gene ID: | 8398 |
| NCBI Accession: | O60733.2 |
| UniProt Secondary Accession: | O60733,O75645, Q8N452, Q9UG29, Q9UIT0, Q9Y671, A8K597 B0QYE8, |
| UniProt Related Accession: | O60733 |
| Molecular Weight: | 46,484 Da |
| NCBI Full Name: | 85/88 kDa calcium-independent phospholipase A2 |
| NCBI Synonym Full Names: | phospholipase A2 group VI |
| NCBI Official Symbol: | PLA2G6 |
| NCBI Official Synonym Symbols: | GVI; PLA2; INAD1; NBIA2; iPLA2; NBIA2A; NBIA2B; PARK14; PNPLA9; CaI-PLA2; IPLA2-VIA; iPLA2beta |
| NCBI Protein Information: | 85/88 kDa calcium-independent phospholipase A2 |
| UniProt Protein Name: | 85/88 kDa calcium-independent phospholipase A2 |
| UniProt Synonym Protein Names: | Group VI phospholipase A2; GVI PLA2; Intracellular membrane-associated calcium-independent phospholipase A2 beta; iPLA2-beta; Patatin-like phospholipase domain-containing protein 9; PNPLA9 |
| Protein Family: | 85/88 kDa calcium-independent phospholipase |
| UniProt Gene Name: | PLA2G6 |
| UniProt Entry Name: | PLPL9_HUMAN |
 | Western blot analysis of extracts of rat liver, using PLA2G6 antibody (CAB9492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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