Anti-PIK3R3 Antibody (CAB17322)
- SKU:
- CAB17322
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PIK3R3 Antibody |
| 抗体コード: | CAB17322 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human PIK3R3 |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | U-251MG, PC-3, 293T |
| 免疫原: | Recombinant protein of human PIK3R3 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 8503 |
| Uniprot: | Q92569 |
| セルラーロケーション: | |
| 計算された分子量: | 43kDa/47kDa/54kDa |
| 観察された分子量: | 60kDa |
| 同義語: | PIK3R3, p55, p55-GAMMA, p55PIK |
| バックグラウンド: | Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity. |
| UniProt Protein Function: | PIK3R3: Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle. Belongs to the PI3K p85 subunit family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Kinase, lipid Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: cytosol; phosphoinositide 3-kinase complex Molecular Function:protein binding; 1-phosphatidylinositol-3-kinase activity; phosphoinositide 3-kinase regulator activity Biological Process: phospholipid metabolic process; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; regulation of phosphoinositide 3-kinase activity |
| NCBI Summary: | Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity. [provided by RefSeq, Jun 2016] |
| UniProt Code: | Q92569 |
| NCBI GenInfo Identifier: | 317373310 |
| NCBI Gene ID: | 8503 |
| NCBI Accession: | Q92569.2 |
| UniProt Related Accession: | Q92569 |
| Molecular Weight: | |
| NCBI Full Name: | Phosphatidylinositol 3-kinase regulatory subunit gamma |
| NCBI Synonym Full Names: | phosphoinositide-3-kinase regulatory subunit 3 |
| NCBI Official Symbol: | PIK3R3 |
| NCBI Official Synonym Symbols: | p55; p55PIK; p55-GAMMA |
| NCBI Protein Information: | phosphatidylinositol 3-kinase regulatory subunit gamma |
| UniProt Protein Name: | Phosphatidylinositol 3-kinase regulatory subunit gamma |
| UniProt Synonym Protein Names: | Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIK |
| UniProt Gene Name: | PIK3R3 |
| UniProt Entry Name: | P55G_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PIK3R3 antibody (CAB17322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (CAB17322) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (CAB17322) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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