Anti-PICALM Antibody (CAB10575)
- SKU:
- CAB10575
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-PICALM Antibody |
| 抗体コード: | CAB10575 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-460 of human PICALM (NP_001008660.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | U-251MG, HepG2, MCF7, HeLa, Mouse lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 290-460 of human PICALM (NP_001008660.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | IKDS TAAS RATT LSNA VSSL ASTG LSLT KVDE REKQ AALE EEQA RLKA LKEQ RLKE LAKK PHTS LTTA ASPV STSA GGIM TAPA IDIF STPS SSNS TSKL PNDL LDLQ QPTF HPSV HPMS TASQ VAST WGGF TPSP VAQP HPSA GLNV DFES VFGN KSTN VIVD SGGF DEL |
| 遺伝子ID: | 8301 |
| Uniprot: | Q13492 |
| セルラーロケーション: | Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus, clathrin-coated pit, clathrin-coated vesicle |
| 計算された分子量: | 59kDa/66kDa/68kDa/70kDa |
| 観察された分子量: | 71kDa |
| 同義語: | PICALM, CALM, CLTH, LAP |
| バックグラウンド: | This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | PICALM: Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Oncoprotein; Vesicle Chromosomal Location of Human Ortholog: 11q14 Cellular Component: AP-2 adaptor complex; cell soma; clathrin coat of coated pit; coated pit; membrane; nucleus; perinuclear region of cytoplasm; postsynaptic membrane; presynaptic membrane; vesicle Molecular Function:clathrin binding; clathrin heavy chain binding; phosphatidylinositol binding; protein binding Biological Process: cell proliferation; clathrin cage assembly; endosome transport; iron ion homeostasis; negative regulation of receptor-mediated endocytosis; positive regulation of transcription, DNA-dependent; protein complex assembly; receptor internalization; receptor-mediated endocytosis; regulation of endocytosis; regulation of protein localization; synaptic vesicle maturation; vesicle-mediated transport Disease: Leukemia, Acute Myeloid |
| NCBI Summary: | This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011] |
| UniProt Code: | Q13492 |
| NCBI GenInfo Identifier: | 116242714 |
| NCBI Gene ID: | 8301 |
| NCBI Accession: | Q13492.2 |
| UniProt Secondary Accession: | Q13492,O60700, Q4LE54, Q6GMQ6, Q86XZ9, B4DTM3, E9PN05 F8VPG7, |
| UniProt Related Accession: | Q13492 |
| Molecular Weight: | 70,037 Da |
| NCBI Full Name: | Phosphatidylinositol-binding clathrin assembly protein |
| NCBI Synonym Full Names: | phosphatidylinositol binding clathrin assembly protein |
| NCBI Official Symbol: | PICALM |
| NCBI Official Synonym Symbols: | LAP; CALM; CLTH |
| NCBI Protein Information: | phosphatidylinositol-binding clathrin assembly protein |
| UniProt Protein Name: | Phosphatidylinositol-binding clathrin assembly protein |
| UniProt Synonym Protein Names: | Clathrin assembly lymphoid myeloid leukemia protein |
| Protein Family: | Phosphatidylinositol-binding clathrin assembly protein |
| UniProt Gene Name: | PICALM |
| UniProt Entry Name: | PICAL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PICALM antibody (CAB10575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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