Anti-Phospho-YAP1-S127 Antibody (CABP0489)
- SKU:
- CABP0489
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Reactivity:
- Monkey
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-Phospho-YAP1-S127 Antibody |
| 抗体コード: | CABP0489 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic phosphorylated peptide around S127 of human YAP1 (NP_001123617.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | NIH/3T3 |
| 免疫原: | A synthetic phosphorylated peptide around S127 of human YAP1 (NP_001123617.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | AHSS P |
| 遺伝子ID: | 10413 |
| Uniprot: | P46937 |
| セルラーロケーション: | Cytoplasm, Nucleus |
| 計算された分子量: | 36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa |
| 観察された分子量: | 68kDa |
 | Western blot analysis of extracts of NIH3T3†’†’€š cells, using Phospho-YAP1-S127 antibody (CABP0489) at 1:1000 dilution.NIH3T3 cells were treated by Sorbitol (0.2M) at 37'C for 60 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-YAP1-S127 Rabbit pAb (CABP0489) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using Phospho-YAP1-S127 Rabbit pAb (CABP0489) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using Phospho-YAP1-S127 Rabbit pAb (CABP0489) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
| 同義語: | COB1, YAP, YAP2, YAP65, YKI, YAP1 |
| バックグラウンド: | This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Code: | P46937 |
| NCBI GenInfo Identifier: | 294862479 |
| NCBI Gene ID: | 10413 |
| NCBI Accession: | P46937.2 |
| UniProt Secondary Accession: | P46937,Q7Z574, B4DTY1, B7ZA01, E3WEB5, E3WEB6, E9PRV2 F5H202, K0KQ18, K0KYZ8, K0L195, K0L1G3, |
| UniProt Related Accession: | P46937 |
| Molecular Weight: | 504 |
| NCBI Full Name: | Transcriptional coactivator YAP1 |
| NCBI Synonym Full Names: | Yes-associated protein 1 |
| NCBI Official Symbol: | YAP1 |
| NCBI Official Synonym Symbols: | YAP; YKI; COB1; YAP2; YAP65 |
| NCBI Protein Information: | transcriptional coactivator YAP1; yorkie homolog; protein yorkie homolog; yes-associated protein 2; 65 kDa Yes-associated protein; yes-associated protein YAP65 homolog |
| UniProt Protein Name: | Transcriptional coactivator YAP1 |
| UniProt Synonym Protein Names: | Protein yorkie homolog; Yes-associated protein YAP65 homolog |
| Protein Family: | Transcriptional coactivator |
| UniProt Gene Name: | YAP1 |
| UniProt Entry Name: | YAP1_HUMAN |