Anti-Phospho-VASP-S239 Antibody (CABP0187)
- SKU:
- CABP0187
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-Phospho-VASP-S239 Antibody |
| 抗体コード: | CABP0187 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding S239 of human VASP |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:1000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding S239 of human VASP |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 7408 |
| Uniprot: | P50552 |
| セルラーロケーション: | Cell junction, Cell projection, Cytoplasm, cytoskeleton, filopodium membrane, focal adhesion, lamellipodium membrane, tight junction |
| 計算された分子量: | 39kDa |
| 観察された分子量: | 50kDa |
 | Western blot analysis of extracts of 293T cells, using Phospho-VASP-S239 antibody (CABP0187) at 1:2000 dilution. 293T cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using Phospho-VASP-S239 Rabbit pAb (CABP0187) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human placenta using Phospho-VASP-S239 Rabbit pAb (CABP0187) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-VASP-S239 Rabbit pAb (CABP0187) at dilution of 1:50 (40x lens). |
| 同義語: | VASP |
| バックグラウンド: | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. |
| UniProt Protein Function: | VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 19q13.32 Cellular Component: filopodium membrane; tight junction; focal adhesion; cytoplasm; plasma membrane; cytosol; actin cytoskeleton Molecular Function:protein binding; actin binding; SH3 domain binding; profilin binding Biological Process: axon guidance; positive regulation of actin filament polymerization; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway; protein homotetramerization |
| NCBI Summary: | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50552 |
| NCBI GenInfo Identifier: | 1718079 |
| NCBI Gene ID: | 7408 |
| NCBI Accession: | P50552.3 |
| UniProt Secondary Accession: | P50552,Q6PIZ1, Q93035, B2RBT9, |
| UniProt Related Accession: | P50552 |
| Molecular Weight: | 380 |
| NCBI Full Name: | Vasodilator-stimulated phosphoprotein |
| NCBI Synonym Full Names: | vasodilator-stimulated phosphoprotein |
| NCBI Official Symbol: | VASP |
| NCBI Protein Information: | vasodilator-stimulated phosphoprotein |
| UniProt Protein Name: | Vasodilator-stimulated phosphoprotein |
| Protein Family: | Vasodilator-stimulated phosphoprotein |
| UniProt Gene Name: | VASP |
| UniProt Entry Name: | VASP_HUMAN |