Anti-Phospho-STAT1 (S727) Antibody (RACO0086)
- SKU:
- RACO0086
- Product type:
- Recombinant Antibody
- Reactivity:
- Human
- Host Species:
- Human
- Isotype:
- IgG
- Application:
- WB
- Application:
- IF
- Application:
- ELISA
- Application:
- IHC
- Conjugation:
- Unconjugated
Description
| 商品名: | Phospho-STAT1 (S727) Antibody |
| Product SKU: | RACO0086 |
| サイズ: | 50ul |
| 宿主種: | Human |
| 申し込み: | ELISA, WB, IHC, IF |
| 推奨される希釈: | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 |
| 反応性: | Human |
| 免疫原: | A synthesized peptide derived from human Phospho-STAT1 (S727) |
| 憲法: | Liquid |
| ストレージバッファ: | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 精製方法: | Affinity-chromatography |
| 抗体のクローン性: | Monoclonal |
| アイソタイプ: | Rabbit IgG |
| Conjugate: | Non-conjugated |
| バックグラウンド: | Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. |
| シノニム: | Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1 |
 | IHC image of RACO0086 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells (treated with 100mM Calyculin A for 30min) with RACO0086 at 1:66,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
 | Western Blot. Positive WB detected in HepG2 whole cell lysate treated with Calyculin A or not). All lanes Phospho-STAT1 antibody at 1.065µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1:50000 dilution. Predicted band size: 87 KDa. Observed band size: 87 KDa. |
| UniProt Protein Function: | STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 2q32.2 Cellular Component: axon; cytoplasm; cytosol; dendrite; nuclear chromatin; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm Molecular Function:double-stranded DNA binding; enzyme binding; identical protein binding; protein binding; protein homodimerization activity; signal transducer activity; transcription factor activity; tumor necrosis factor receptor binding Biological Process: apoptosis; blood circulation; defense response to virus; endothelial cell migration; JAK-STAT cascade; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of viral protein levels in host cell; positive regulation of mesenchymal cell proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; response to cAMP; response to cytokine stimulus; response to peptide hormone stimulus; transcription, DNA-dependent; tumor necrosis factor-mediated signaling pathway; viral reproduction Disease: Immunodeficiency 31a; Immunodeficiency 31b; Immunodeficiency 31c |
| NCBI Summary: | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P42224 |
| NCBI GenInfo Identifier: | 2507413 |
| NCBI Gene ID: | 6772 |
| NCBI Accession: | P42224.2 |
| UniProt Secondary Accession: | P42224,Q53S88, Q53XW4, Q68D00, Q9UDL5, A8K989, B2RCA0 D2KFR8, D3DPI7, |
| UniProt Related Accession: | P42224 |
| Molecular Weight: | |
| NCBI Full Name: | Signal transducer and activator of transcription 1-alpha/beta |
| NCBI Synonym Full Names: | signal transducer and activator of transcription 1 |
| NCBI Official Symbol: | STAT1 |
| NCBI Official Synonym Symbols: | CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91 |
| NCBI Protein Information: | signal transducer and activator of transcription 1-alpha/beta |
| UniProt Protein Name: | Signal transducer and activator of transcription 1-alpha/beta |
| UniProt Synonym Protein Names: | Transcription factor ISGF-3 components p91/p84 |
| Protein Family: | Signal transducer and activator of transcription |
| UniProt Gene Name: | STAT1 |
| UniProt Entry Name: | STAT1_HUMAN |