Anti-Phospho-Smad1-S463/465 + Smad5-S463/465 + Smad9-S465/467 pAb Antibody (CABP0850)
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- SKU:
- CABP0850
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-Phospho-Smad1-S463/465 + Smad5-S463/465 + Smad9-S465/467 Antibody |
| 抗体コード: | CABP0850 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding S463 of human Smad1 |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HeLa |
| 免疫原: | A phospho specific peptide corresponding to residues surrounding S463 of human Smad1 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 4086/4090/4093 |
| Uniprot: | Q15797/Q99717/O15198 |
| セルラーロケーション: | |
| 計算された分子量: | 60kDa |
| 観察された分子量: | 60kDa |
 | Western blot analysis of extracts of HeLa cells, using Phospho-Smad1(Ser463/465)/Smad5(Ser463/465)/Smad9(Ser465/467) pAb (CABP0850) at 1:1000 dilution.HeLa cells were treated by ATP(5 mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
| 同義語: | Smad1/Smad5/Smad9 |
| バックグラウンド: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |